To detect the expression of eNOS、iNOS in mRNA and protein in kidney and heart on salt-sensitive hypertensive rats induced by sensory denervation,to analyse the relationship between the expression of eNOS and salt-sen...
详细信息
To detect the expression of eNOS、iNOS in mRNA and protein in kidney and heart on salt-sensitive hypertensive rats induced by sensory denervation,to analyse the relationship between the expression of eNOS and salt-sensitive hypertension,and the relationship between the expression of iNOS and heart,kidney apoptosis,and to propose the formation of salt-sensitive hypertension and heart,kidney cell *** Wistar rats were given 50 mg/ kg capsaicin subcutaneously on the 1st and 2nd day of *** weanling period,male rats were divided into 4 gruops and fed different salt diets for 4 *** rats were not treated with capsaicin and receive a normal diet(containing 0.5%NaCl).Tail-cuff systolic blood pressure,body weight, kidney and heart histopathology,iNOS activity,NO content,eNOS and iNOS protein,eNOS and iNOS mRNA,level of cell apoptosis were examined,using pathology hematoxylin-eosin staining, immunohistochernistry,RT-PCR,single cell gel *** study provides that neonatal treatment with capsaicin led to the elevation of blood pressure in rats fed a high salt diet (containing 4%NaCl).The decreased express of eNOS mRNA and protein was related with the formation of salt-sensitive *** increased express of iNOS mRNA and protein could produce a large number of NO in heart and *** can increase the apoptosis and the damage of in salt-sensitive hypertensive rats of heart and *** data also suggest that neonatal capsaicin treatment may impair renal and heart in response to high sodium intake.
暂无评论