Objective: To investigate the mechanism of BUEA3 A affects the biological behavior of breast cancer cells through ***: Breast cancer tissues and adjacent tissues of 172 patients were collected to investigate relations...
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Objective: To investigate the mechanism of BUEA3 A affects the biological behavior of breast cancer cells through ***: Breast cancer tissues and adjacent tissues of 172 patients were collected to investigate relationship between UBE3 A and clinicopathological features of breast cancer patients. Biological behavior of MDA-MB-231 cells were detected in vivo and in vitro experiments after knock-down UBE3 A expression. Changed genes and proteins were detected by transcriptome sequencing and 2-Directional electrophoresis(2-DE) experiments and protein tandem mass spectrometry before and after UBE3 A knock down. ANXA2 expression were detected by WB and ICH after knocking-down UBE3 A expression. Subcellular location of ANXA2 were observed by ***: UBE3 A was upregulated in breast cancer tissues, as well as in particular pathological tissues,including large-sized tumor, invasive, lymph node metastasis, high clinical stage, HER2-, Ki-67+ and triple negative breast cancer. UBE3 A knockdown caused cells S phase arrest, apoptosis and decrease of cell proliferation, invasion and metastasis;In vivo, breast cancer cells' metastasis decreased significantly with UBE3 A knock-down. Interestingly, ANXA2 consistently appeared in transcriptome sequencing, gene expression profiling and tandem mass spectrometry by observing 44 genes and 24 proteins. We also identified that UBE3 A knockdown in breast cancer induced ANXA2 migration from membrane to cytoplasm, which may benefit for further clarify the mechanism of breast ***: In conclusion, our results indicate that UBE3 A expression is closely correlated with clinicopathological characteristics of breast cancer and MDA-MB-231 cells' proliferation, invasion and metastasis. UBE3 A also regulates expression and subcellular location of ANXA2.
Objective: To investigate the mechanism of BUEA3A affects the biological behavior of breast cancer cells through ***: Breast cancer tissues and adjacent tissues of 172 patients were collected to investigate relationsh...
详细信息
Objective: To investigate the mechanism of BUEA3A affects the biological behavior of breast cancer cells through ***: Breast cancer tissues and adjacent tissues of 172 patients were collected to investigate relationship between UBE3A and clinicopathological features of breast cancer *** behavior of MDA-MB-231 cells were detected in vivo and in vitro experiments after knock-down UBE3A expression.
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