Objective To study the selective toxicity of immunotoxin (IT) on T cells in cord blood and simultaneously determine its effect on hematopoietic progenitor cells Methods The percentage of CD 5 and CD 8 T c...
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Objective To study the selective toxicity of immunotoxin (IT) on T cells in cord blood and simultaneously determine its effect on hematopoietic progenitor cells Methods The percentage of CD 5 and CD 8 T cell subsets in cord blood (CB) and bone marrow (BM) as well as peripheral blood (PB) was measured by immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti alkaline phosphatase (APAAP complexes) One way mixed lymphocyte cultures (MLC) were performed to compare the proliferative response of CB with that of PB The proliferative capability of cord blood T cells and T lymphocyte transformation capacity were evaluated in the presence of anti CD 8 or anti CD 5 immunotoxin by one way MLC and colorimetric MTT (tetrazolium) assay, respectively The effect of IT on the growth of hematopoietic progenitor cell of colony forming unit granulocyte and macrophage (CFU GM), burst forming unit erythroid(BFU E), multipotential hemotapoietic progenitors (CFU Mix) from CB were estimated by colony forming assays Results A certain proportion of CD 5 and CD 8 T cells existed in CB The alloproliferative capacity of CB was similar to that of PB CD 5: Ricin at a dosage of 1×10 10 -1×10 8 mmol/L and CD 8: Ricin concentration in the range of 1×10 9 -1×10 8 mmol/L effectively decreased both the proliferative capability of T cells in MLC during CB and T cell transformation Over the dosage of 1×10 10 -1×10 9 mmol/L, both kinds of IT didn't obviously affect the growth of hematopoietic progenitor cells Conclusion CD 5: Ricin and CD 8: Ricin may effectively deplete T cells and may not significantly inhibit the function of hemaptopoietic cells at a specific dosage
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