[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.[Method] DNA fingerprints of ***,*** and *** were established by using ISS...
详细信息
[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.[Method] DNA fingerprints of ***,*** and *** were established by using ISSR-PCR *** Analysis was carried out by using UPGMA method based on Nei＇s genetic distances among each individual.[Result] Six polymorphic primers were selected from 50 ISSR primers for ISSR-PCR amplification,and totally 86 discernible DNA bands were amplified with 53 polymorphic bands,accounting for 61.2% of the *** average number of DNA bands amplified by each primer was ***,totally 5 primers had amplified differential bands and specific bands,which were able to accurately identify ***,*** and *** calculated by DPS v3.01 software,the genetic distances among test materials were ranged from 0.166 67 to 0.809 52,with an average of 0.563 57.[Conclusion] ISSR-PCR method can be used to identify ***,*** and *** effectively.
暂无评论