铁死亡是一种新发现的程序性细胞死亡形式,其特征是铁过载及脂质过氧化。越来越多的证据表明,铁死亡与妊娠相关疾病的发生密切相关有关,而抑制铁死亡对于妊娠相关疾病的治疗具有一定作用。本综述总结了铁死亡发生的分子机制及铁死亡在妊娠相关疾病中的最新研究进展,期望对妊娠相关疾病的诊治带来新的思路。Ferroptosis is a newly discovered form of programmed cell death characterized by iron overload and lipid peroxidation. Accumulating evidence suggests a significant association between ferroptosis and the pathogenesis of pregnancy-related disorders. Studies have shown that inhibiting ferroptosis may offer therapeutic benefits in managing these conditions. This review aims to elucidate molecular mechanisms underlying ferroptosis and to summarize recent advancements in understanding its role in pregnancy-related diseases, thereby providing novel insights for the diagnosis and treatment of such disorders.
目的 探究癌-睾丸抗原顶体素结合蛋白(acrosin binding protein,ACRBP)在肝癌中的表达及其与免疫浸润的相关性。方法 首先利用UALCAN(university of alabama at birmingham cancer data analysis portal,UALCAN)和HPA(human protein atl...
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目的 探究癌-睾丸抗原顶体素结合蛋白(acrosin binding protein,ACRBP)在肝癌中的表达及其与免疫浸润的相关性。方法 首先利用UALCAN(university of alabama at birmingham cancer data analysis portal,UALCAN)和HPA(human protein atlas,HPA)数据库分析肝癌和正常肝组织中ACRBP的mRNA和蛋白的表达情况。基于TCGA数据,采用TIMER(tumor immune estimation resource,TIMER)数据库、CIBERSOFT(cell-type identification by estimating relative subsets of rna transcripts,CIBERSORT)工具和TISIDB(an integrated repository portal for tumor-immune system interactions,TISIDB)数据库分析肝癌中ACRBP的表达与免疫细胞浸润及免疫调节基因的相关性。此外,还分离人外周血单个核细胞(peripheral blood mononuclear cell,PBMC),与ACRBP表达下调的肝癌细胞共培养,通过ELISA(enzyme linked immunosorbent assay,ELISA)检测免疫分子的分泌,流式细胞术检测PBMC的凋亡情况。结果 生物信息学分析显示,在肝癌组织中,ACRBP mRNA及蛋白表达均显著高于正常肝组织;ACRBP的mRNA表达与调节性T细胞(Treg细胞)浸润、免疫抑制分子和趋化因子等多种免疫调节基因呈正相关;与正常肝组织比较,ACRBP高表达的肝癌组织中浆细胞,γδT细胞,自然杀伤细胞(NK细胞)和M2型巨噬细胞浸润比例明显减少。在体外试验中,下调肝癌细胞中ACRBP的表达,发现PBMC分泌α肿瘤坏死因子、γ干扰素和白细胞介素2增加,PBMC的凋亡率有所增多。结论 肝癌中ACRBP的表达与免疫细胞浸润相关,ACRBP参与免疫细胞调节而影响肿瘤微环境,为将来开发以ACRBP为靶标的免疫治疗提供了理论基础。
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