感染性疾病发病率、死亡率仍位居全球前列。早期、精准明确致病病原体对该疾病临床工作诊治尤为关键。传统病原学检测技术耗时长,且敏感度及特异度均低,容易遗漏某些常见病原体。因此为更好满足当今感染性疾病诊治的临床需求,需要寻找更敏感、高效、适用性强的病原学检测方法。靶向二代测序技术相对于传统检测技术而言,具有通量高、耗时短、无偏倚、成本较低等独特优势,能真实获得检出病原体的拷贝数,在感染性疾病诊断中具有重要参考价值。本文就靶向二代测序技术在感染性疾病诊断中应用进行综述。The incidence rate and mortality rate of infectious diseases are still among the highest in the world. Early and accurate identification of the pathogenic pathogen is particularly critical for clinical diagnosis and treatment of the disease. Traditional etiological detection techniques are time-consuming, with low sensitivity and specificity, and are prone to missing certain common pathogens. Therefore, in order to better meet the clinical needs of diagnosis and treatment of infectious diseases, it is necessary to find more sensitive, efficient, and applicable etiological detection methods. Compared with traditional detection technology, targeted next-generation sequencing technology has unique advantages such as high throughput, short time consumption, no bias, and low cost. It can truly obtain the copy number of pathogens detected, and has important reference value in the diagnosis of infectious diseases. This article reviews the application of targeted next-generation sequencing technology in the diagnosis of infectious diseases.
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