ABC1(Activity of bc1 complex)家族属于蛋白质激酶家族,其成员普遍存在于原核和真核生物中。已有研究表明,几个植物ABC1基因参与非生物胁迫应答。为了解ABC1基因在水稻中的结构和功能,采用生物信息学方法分别在水稻和拟南芥上鉴定出15...
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ABC1(Activity of bc1 complex)家族属于蛋白质激酶家族,其成员普遍存在于原核和真核生物中。已有研究表明,几个植物ABC1基因参与非生物胁迫应答。为了解ABC1基因在水稻中的结构和功能,采用生物信息学方法分别在水稻和拟南芥上鉴定出15个和17个ABC1基因,并进行了系统发育和表达分析。结果表明,该家族在单、双子叶植物分离之前就已经发生了分化,其基本特征已经形成;单、双子叶植物分离之后,该家族在水稻和拟南芥中均以物种特异的方式进行了扩增。内含子/外显子结构分析显示多数直系同源基因之间外显子大小接近,而内含子差别较大,水稻含有更多大的内含子;内含子获得是近期伴随水稻ABC1家族进化的重要事件。多序列比对显示,ABC1结构域具有1个保守的氨基酸片段和4个保守的氨基酸残基。在线亚细胞定位预测9个水稻ABC1蛋白定位在叶绿体上。实时定量RT-PCR分析表明,水稻ABC1基因主要在叶片中表达,并且受多种非生物胁迫因素包括H2O2、脱落酸、低温、干旱、黑暗和高盐的调控。说明水稻ABC1家族不仅在逆境胁迫应答中发挥重要作用,可能还与水稻特定的生理过程有关。
Through constructing the F-2 clonal population of an intercross,Teqing/Lemont,two quantitative trait loci(QTLs),qSB-9 and qSB-11,contributing to sheath blight resistance were preliminarily ***,from the F-2 clonal popu...
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Through constructing the F-2 clonal population of an intercross,Teqing/Lemont,two quantitative trait loci(QTLs),qSB-9 and qSB-11,contributing to sheath blight resistance were preliminarily ***,from the F-2 clonal population of Teqing/Lemont,a double-susceptible and two double-resistant clonal lines was selected as pure parents and were separately intercrossed and backcrossed to recurrent parents,Teqing and *** BC-1F-1,marker-assisted selection was conducted and all backcrossed plants were inoculated by short toothpicks incubated with a strain,RH-9 of Rhizoctonia solani for identification of the *** BC-2F-1,according to the similarity of integrative traits between backcrossed plants and their recurrent parents,we gathered BC-2F-2 seeds of some ***-2F-2 plants of coinciding requirement were selected through marker-assisted selection during the period of seedling stage,and separately mixed as homozygous ***,double-susceptible lines in the background of Teqing,double-susceptible and double-resistant lines in the background of Lemont were *** results of the inoculation test suggested that method of molecular marker-assisted selection for sheath blight resistance with investigation of disease ratings in backcross generations can quickly check up the reliability of location of resistant QTL and confirm the QTL interval on chromosome.
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