Mesenchymal-epithelial transition(MET)occurs commonly in both physiological and pathological ***:To observe MET-associated alteration during the trans-differentiation from MSCs to neuron-like cells,and to explore the ...
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Mesenchymal-epithelial transition(MET)occurs commonly in both physiological and pathological ***:To observe MET-associated alteration during the trans-differentiation from MSCs to neuron-like cells,and to explore the possible molecular ***:Bone marrow MSCs were isolated from rat femur and purified in continuous cell *** induced differentiation to neuron-like cells by the combination of butylated hydroxyanisole(BHA)and dimethyl sulfoxide(DMSO),cells were tested by comparative polymerase chain reaction(PCR)for the relative expression of MET biomarkers and transcription factors,and for cell cycle by flow ***,target genes of Wnt/β-catenin pathway were also analyzed by comparative PCR to determine the possible ***:in MSC-induced neuron-like cells,MET-associated transcription factors such as Snail,Slug,ZEB1,ZEB2,and Twist were significantly attenuated in expression *** marker vimentin was *** protein E-cad was slightly down-regulated,while N-cad level was marginally *** of proliferating cells(S phase in cell cycle)markedly shrank from 40.42%for MSCs to 6.76%for MSC-derived ***,Wnt/β-catenin target genesβ-catenin and c-Myc were decreasingly ***:chemically induced trans-differentiation from MSC to neuron caused similar MET-featured alteration in gene expression and proliferation to known MET,which might be underlied by de-activation of Wnt/β-catenin pathway.
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