叶片结构性状是植物适应环境和维持生命活动的基础。为探究荒漠建群种灰杨(Populus pruinosa Schrenk)叶片结构性状的空间变异及其与环境因子的关系,以新疆11个天然灰杨居群为研究对象,对灰杨叶片的形态、解剖性状进行研究,用Pearson相关性分析、植物性状网络(PTN)、冗余分析(RDA)等方法对灰杨叶片结构性状的空间变异与气候、土壤因子的关系进行分析。结果表明:1) 灰杨叶片的解剖性状比形态性状的变异性更高,出叶强度(LIM)和主脉维管束面积(MVBA)具有较高的变异系数。2) 形态性状和解剖性状间存在显著的协同与权衡关系,在性状网络中,MVBA为中心性状,可能起着影响灰杨叶片结构的中心调控作用。3) 年均温、年降水量和土壤全钾含量对灰杨叶片结构性状的影响较大,在较干旱生境下灰杨表现出小比叶面积和大叶组织密度的资源保守型策略,相对湿润时则采取高比叶面积和低叶组织密度的资源获取型策略。Leaf structural traits are fundamental to plant adaptation and the maintenance of life processes. To investigate the spatial variation in leaf structural traits of the desert tree species Populus pruinosa Schrenk and its association with environmental factors, 11 natural P. pruinosa populations in Xinjiang were selected as the research objects to study the morphology and anatomical traits of P. pruinosa leaves. Pearson correlation analysis, plant trait network, redundancy analysis and other methods were performed to analyze the spatial variation in leaf traits and their relationship with climatic and soil factors. The results showed that: 1) The leaf anatomical traits of P. pruinosa exhibited greater variability than their morphological traits, and leafing intensity and main vein vascular bundle area showed particularly high coefficients of variation. 2) A significant synergistic and trade-off relationship was identified between morphological and anatomical traits, with main vein vascular bundle area emerged as a central trait within the structural trait network. This suggested a pivotal regulatory role in shaping the leaf structure of P. pruinosa. 3) The annual average temperature, annual precipitation, and soil total potassium content significantly influenced the leaf structural traits of P. pruinosa. In drier habitats, P. pruinosa exhibited a resource conservation strategy of small SLA and large LTD, whereas in relatively humid environments, an opposite resource acquisition strategy was adopted.
【目的】研究苦苣菜水提物对脂多糖(LPS)诱导的小鼠肺炎模型中炎症相关因子及炎症信号通路的影响,探究苦苣菜水提物对小鼠肺炎的抗炎作用。【方法】制备苦苣菜水提物,将昆明小鼠随机分为空白组、模型组、阳性对照组及苦苣菜水提物低、中、高浓度组,空白组小鼠不做任何处理,模型组小鼠灌胃0.3 mL生理盐水,阳性对照组小鼠灌胃0.3 mL 5 mg/kg地塞米松,苦苣菜水提物低、中、高浓度组小鼠依次灌胃0.3 mL 100、200和400 mg/mL苦苣菜水提物,持续灌胃给药1周后,空白组小鼠腹腔注射0.3 mL生理盐水,其余组小鼠腹腔注射0.3 mL 30 mg/kg LPS诱导建立小鼠肺炎模型。采用ELISA方法检测各组小鼠血清中白细胞介素-6(IL-6)、IL-10、转化生长因子-β(TGF-β)的含量;利用免疫组织化学(IHC)和Western blotting检测各组小鼠肺中炎症信号通路(HMGB1/TLR4/NF-κB)蛋白的表达水平。【结果】与空白组相比,模型组小鼠血清中IL-6含量显著上调(P<0.05),IL-10、TGF-β含量有所上调,肺脏中HMGB1、TLR4、NF-κB的分布及蛋白表达量极显著上调(P<0.01);与模型组相比,苦苣菜水提物处理组小鼠血清中促炎因子IL-6含量有所下调,抗炎因子IL-10含量有所上调,抗炎因子TGF-β含量极显著上调(P<0.01);在肺脏中HMGB1、TLR4、NF-κB大量分布在肺泡周围的上皮细胞上,与模型组相比,苦苣菜水提物处理组小鼠肺脏中HMGB1、TLR4、NF-κB的分布极显著下调(P<0.01),且表现出剂量依赖效应,HMGB1、TLR4蛋白表达量极显著下调(P<0.01),NF-κB蛋白表达量显著或极显著下调(P<0.05;P<0.01)。【结论】苦苣菜水提物能抑制LPS诱导的肺炎小鼠血清中促炎因子IL-6的分泌及肺脏中信号通路HMGB1、TLR4、NF-κB蛋白的表达,促进小鼠血清中抗炎因子IL-10、TGF-β的分泌,表明苦苣菜水提物具有抗肺炎作用。
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