目的探讨低密度脂蛋白相关蛋白5(LRP5)基因多态性及突变与绝经后2型糖尿病(type 2diabetic,T2DM)女性伴骨质疏松症(osteoporosis,OP)的关系,为绝经后女性T2DM合并OP的防治提供依据。方法纳入136例新疆绝经后女性,依据T2DM病史或OGTT分为NGT组(A组=26例)、骨量异常组(B组=28例)、T2DM组(C组=27例)和T2DM伴骨量异常组(D组=55例);记录年龄、身高等基线资料;检测空腹血糖(FPG)、钙(Ca)、磷(P)等生化指标;采用双能X线(dual energy x-ray,DEXA)法测定骨密度(bone mineral density,BMD);LRP5基因位点的多态性的检测采用飞行时间质谱法。结果 (1)在LRP5基因rs901825位点中,与A组相比,D组基因型分布差异具有统计学意义(P <0.001);在LRP5基因rs7125942位点中,与A组相比,B组基因型分布差异具有统计学意义(P=0.038)。(2)在D组中,LRP5基因rs7125942位点,GC基因型(突变型)的TG高于CC基因型(野生型)[(4.12±0.79)vs.(3.22±1.04),P=0.006]。(3)多元线性回归分析显示:TG(β=0.037,P=0.008)与BMD(L1-4)的水平呈正相关,绝经年限(β=-0.007,P=0.002 5)与BMD(L1-4)的水平呈负相关;绝经年限(β=-0.008,P=0.028)与BMD(股骨颈)的水平呈负相关。结论(1)新疆绝经后女性LRP5基因rs901825、rs7125942位点基因多态性及基因频率分布与骨代谢可能有关;(2)LRP5基因rs7125942位点的基因突变可能与新疆绝经后女性脂代谢有关;(3)在绝经后T2DM女性中,TG的降低和绝经年限的增高是BMD降低的危险因素。
目的:探讨骨保护素(osteoprotegerin, OPG)基因rs2073618、rs3102735位点多态性及突变与绝经后2型糖尿病(Type 2 diabetes mellitus, T2DM)女性骨代谢的关系,为后续治疗提供依据。方法:1) 选取石河子地区研究对象200例,所有研究对象均为绝经后女性,根据骨密度(Bone mineral density, BMD)分为:正常对照组(A组)、单纯骨量异常组(B组),根据不同的糖耐量水平分为:单纯T2DM (C组)、T2DM伴骨量异常组(D组)。2) 测定骨、糖、脂、糖化血红蛋白(Glycated hemoglobin, HbA1c)、BMD (L1-4) 及BMD (股骨颈)等相关指标。3) 运用Mass ARRAY质谱仪检测OPG基因位点多态性。4) 多组间计量资料采用方差分析;BMD影响因素采用多元线性回归分析。结果:1) 与A组相比,C组、D组的空腹血糖(FPG)及HbAlc水平升高;D组、B组BMD (L1-4)及BMD (股骨颈)水平降低,(P Objective: To investigate the relationship between rs2073618 and rs3102735 polymorphism and mutation of osteoprotegerin (OPG) gene and bone metabolism in postmenopausal women with Type 2 diabetes mellitus (T2DM), so as to provide evidence for follow-up treatment. Methods: 1) A total of 200 subjects were selected from Shihezi area, all of which were postmenopausal women. According to Bone mineral density (BMD), the subjects were divided into normal control group (group A) and simple abnormal bone mass group (group B), and according to different glucose tolerance levels, they were divided into: simple T2DM (group C) and T2DM with abnormal bone mass (group D). 2) Measurement of bone, sugar, lipid, Glycated hemoglobin (HbA1c), BMD (L1-4), and BMD (neck of femur), respectively. 3) The polymorphism of OPG gene locus was detected by Mass ARRAY mass spectrometer. 4) Analysis of variance was used for measurement data among multiple groups;the influencing factors of BMD were analyzed by multiple linear regression. Results: 1) Compared with group A, fasting blood glucose (FPG) and HbAlc levels in groups C and D were increased. BMD (L1-4) and BMD (neck of femur) levels in groups D and B were decreased (P < 0.05). 2) In group D, Ca of rs2073618 mutant (GG/GC) was higher than that of wild type (CC), and Ca of rs3102735 mutant (CC/TC) was lower than that of wild type (TT). In group C, BMD (1-4) of rs2073618 mutant (GG/GC type) was higher than that of wild type (CC type), P < 0.05. 3) Multiple linear regression analysis: the in
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