Ocular toxoplasmosis(OT),a potentially blinding disorder,is the most common cause of human retinochoroiditis *** this study,the role of CD8+,CD4,B cell,and IL-10 gene in the protective immunity of ocular vaccination w...
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Ocular toxoplasmosis(OT),a potentially blinding disorder,is the most common cause of human retinochoroiditis *** this study,the role of CD8+,CD4,B cell,and IL-10 gene in the protective immunity of ocular vaccination with the temperature-sensitive mutant of RH Toxoplasma gondii strain(ts-4) and challenge with RH strain was investigated in murine models utilizing inbred C57BL/6 mice-deficient in CD4,CD8,B cells (μMT),or IL-10 ***-normal histology with few tachyzoites was observed in the eyes of wild-type(WT) mice immunized intraperitoneally with the avirulent ts-4 strain followed by intraocular challenge with the RH strain of *** contrast,all mutant mice had partially decreased vaccine-induced resistance associated with increased ocular parasite burdens after RH strain *** inflammation without evidence of necrosis associated with increased parasite burdens were observed in the eyes of CD4 knockout(KO) mice after ***8 KO andμMT mice,as well as IL-10 KO mice,demonstrated increased ocular necrosis in response to ocular *** parasite burden was significantly increased in the eyes of both CD8 KO andμMT mice.A significant increase of the percentages of B cells and CD8 T cells in the draining lymph nodes were observed in WT and IL-10 KO mice after *** expected,there were no increases in the levels of immunoglobulin G in serum or aqueous humor inμMT mice,and there was no increase in the levels of gamma interferon and tumor necrosis factor alpha in the sera of CD4 KO mice after *** results suggest that the attenuated ts-4 of *** inoculated intracamerally can induce ocular protective immunity;mice deficient in CD8,CD4 T cells or B cells exhibit increased ocular parasite burden after challenge;CD4,CD8,B cell, and IL-10 gene are all necessary to the vaccine-induced resistance to ocular challenge by virulent RH strain.
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