MicroRNA (miRNA) is a family of 19-24 nucleotides small non-coding RNA in eukaryotic organisms,which can regulate genes at the post-transcriptional level. miRNA has been shown to be involved in a variety of cellular p...
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MicroRNA (miRNA) is a family of 19-24 nucleotides small non-coding RNA in eukaryotic organisms,which can regulate genes at the post-transcriptional level. miRNA has been shown to be involved in a variety of cellular processes such as development,proliferation,differentiation,apoptosis etc,and is closely related to the oncogenesis. Recent study indicates that several miRNAs play an important role in initiation and progression of hematological malignancies. miRNA expression profiling has identified signatures associated with diagnoses,prognoses and therapy of hematological malignancies. The review discusses miRNA and its biogenesis,functional mechanisms and potential role in the diagnosis,prognoses and therapy of hematological malignancies.
目的探讨血管紧张素-(1-7)[Ang-(1-7)]对血管紧张素Ⅱ(AngⅡ)致内皮细胞损伤的保护作用。方法体外培养的ECV-304内皮细胞株,随机分为4组:(1)对照组:单纯DMEM培养基培养;(2)AngⅡ组:单纯DMEM培养基,加入AngⅡ,终浓度为10-7mol/L;(3)Ang-(1-7)组:单纯DMEM培养基,加入Ang-(1-7),终浓度为10-7mol/L;(4)混合组:单纯DMEM培养基,加入Ang-(1-7)预处理30 m in,再加入AngⅡ,两者终浓度均为10-7mol/L。各组作用6,12,24 h,倒置相差显微镜下观察各时间点细胞的形态变化,收集细胞,流式细胞仪测定细胞内的活性氧,硝酸还原酶法测定上清液中的一氧化氮。结果AngⅡ组内皮细胞发生损伤性的形态学变化,活性氧生成增加,NO生成下降,与其他3组比较差异有显著性;Ang-(1-7)组与对照组比较,细胞形态没有变化,活性氧和NO含量差异无显著性;混合组与AngⅡ组相比,细胞损伤明显减弱,活性氧和NO含量与AngⅡ组比较差异有显著性,与对照组比较差异无显著性。结论Ang-(1-7)对AngⅡ导致的内皮损伤有保护性作用。
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