An experiment was conducted to compare the effects of two mouse thrombocytopenia models induced by cyclophosphamide at two different administration routes to determine a proper cyclophosphamide administration route th...
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An experiment was conducted to compare the effects of two mouse thrombocytopenia models induced by cyclophosphamide at two different administration routes to determine a proper cyclophosphamide administration route that could cause stable thrombocytopenia. A suitable drug dosage that could induce thrombocytopenia in mouse efficiently with the definite administration route was then investigated. BALB/c mice were randomly divided into Normal, Model A and Model B groups. To Model A, 200 mg/kg of cyclophosphamide was given by vena caudalis injection as first dose and 30 mg/kg as maintenance dose by intraperitoneal injection at the following 6 days. To Model B, 150 mg/kg of cyclophosphamide was given by subcutaneous injection once a day for consecutive 3 days. All groups were under investigation for 15 days. The result suggested that a decrease in the number of blood platelets of Model B at the 7th day were significantly than that of Normal. Other platelet related indices like platelet distribution width, mean platelet volume and platelet-large cell ratio of Model B increased significantly in comparison with those of Normal group. The platelets count was reduced but fluctuated greatly, and more than half of the mice died in Model A. Therefore, subcutaneous injection of cyclophosphamide for 3 days was used for the cyclophosphamide dosage test. BALB/c mice were randomly divided into Normal, cyclophosphamide low dose （100 mg/kg）, medium dose （120 mg/kg） and high dose （140 mg/kg） groups. All groups were under investigation for 11 days. Though all 3 dosages successfully initiated thrombocytopenia as the platelets number dropped at the 7th day, the low dose was considered to be a suitable one that was of high efficacy and low toxicity. Thus, BALB/c mice challenged by subcutaneous injection of cyclophosphamide 100 mg/kg per day for 3 consecutive day is one simple, feasible and stable mouse thrombocytopenia model that could be used for pharmacodynamic test of the drugs which are
真菌是新颖结构及活性次生代谢产物的重要来源。前期研究中我们发现一株红树林真菌（094811）"泡盛酒曲霉"显示强的细胞毒活性,从该菌株中我们曾报道了一系列新的多羟基甾醇的脂肪酸酯（Gao et al.2007）。本研究报道从该菌...
详细信息
真菌是新颖结构及活性次生代谢产物的重要来源。前期研究中我们发现一株红树林真菌（094811）"泡盛酒曲霉"显示强的细胞毒活性,从该菌株中我们曾报道了一系列新的多羟基甾醇的脂肪酸酯（Gao et al.2007）。本研究报道从该菌株活性部位中得到的两个新的多羟基甾类成分（1和2）。这两个新化合物的结构通过各种波谱学方法鉴定为22E-7α-甲氧基-5α,6α-环氧麦角甾-8（14）,22-二烯-3β-醇（1）和22E-3β-羟基-5α,6α,8α,14α-二环氧麦角甾-22-烯-7-酮（1）。化合物1和2在DMSO-d和CDCl两种溶剂中的氢谱、碳谱数据被报道,并被全归属。化合物1和2抑制A549细胞增殖的活性被评价(化合物1的IC:70μM;化合物2的IC:64μM)。并且,基于NOE分析,对化合物1中B环的构象进行了详细的研究。化合物1的B环属于环氧环己烷体系。一般的环氧环己烷体系都是以半椅式构象存在（Abraham et al.1 999;Pawar and Noe 1 998;Samoshin et al.1996;Traetteberg et al.1980）,但化合物1的B环则以内-船式构象存在,对于这一特殊构象我们归因于结构中C-8/C-14双键的存在。虽然,5α,6α-环氧甾醇的结构较为普遍,但关于其B环构象的深入研究未见报道。本研究的发现丰富了对小环体系构象的认识,具有重要的理论价值。
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