Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded ...
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Germin and Germin-like protein （GLP） have various proposed roles in plant developmental stages and stress- related processes. A novel GLP cDNA clone was isolated from a cDNA library of Tamarix hispida. ThGLP, coded 225aa, possesses conserved motif of plant germin and Germin-like protein. ThGLP belongs to true germin subfamily through phylogenetic analyses. Gene expression profiles in roots and leaves were evaluated using real-time quantitative RT-PCR. The results show that the gene was highly induced by drought, salt, low temperature, CdCl2 and abscisic acid treatments. Our results demonstrate that the ThGLP gene is expressed in leaves and roots, is involved in different abiotic stress re-sponses and controlled by an ABA-dependent signaling pathway.
In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus *** the present study,the expression level of callose synthase gene in embryos of ***...
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In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus *** the present study,the expression level of callose synthase gene in embryos of *** in response to 2,4-D,sucrose and mannitol treatments was analyzed by *** results show that plasmolysis pretreatment using sucrose and mannitol significantly promoted the expression of callose synthase ***,the thicker cell walls of explant plasmolyzed compared with controls were observed during the somatic *** suggest that the callose may make the cells in epidermis separate from neighboring cells and then develop into embryogenic potential cells.
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