粘膜是人体免疫的第一道屏障,分泌型 IgA 在粘膜免疫防御中起主要作用, 但目前直接通过细胞融合制备 IgA 分泌型的杂交瘤细胞很困难,影响了对 IgA 功能机制的研究。因 IgG 分泌型细胞有一定几率自然转变为 IgA 分泌型细胞,我们利用有限...
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粘膜是人体免疫的第一道屏障,分泌型 IgA 在粘膜免疫防御中起主要作用, 但目前直接通过细胞融合制备 IgA 分泌型的杂交瘤细胞很困难,影响了对 IgA 功能机制的研究。因 IgG 分泌型细胞有一定几率自然转变为 IgA 分泌型细胞,我们利用有限稀释法,对麻疹病毒 M 蛋白特异的 IgG 分泌型杂交瘤细胞系通过类别
The first HIV-1 epidemic in China was found among male injection drug users (IDUs) in the Dehong prefecture of Yunnan in *** then, Yunnan province has had the highest numbers of HIV-1 infections and AIDS-related death...
The first HIV-1 epidemic in China was found among male injection drug users (IDUs) in the Dehong prefecture of Yunnan in *** then, Yunnan province has had the highest numbers of HIV-1 infections and AIDS-related deaths in *** is also considered a site for future vaccine studies, but a systematic study of the molecular epidemiology of HIV-1 in this province has not been *** goal of this study is to conduct a molecular characterization of HIV-1 throughout every region of Yunnan.
The first HIV-1 epidemic in China was found among male injection drug users （IDUs） in the Dehong prefecture of Yunnan in 1989. Since then, Yunnan province has had the highest numbers of HIV-1 infections and AIDS-rel...
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The first HIV-1 epidemic in China was found among male injection drug users （IDUs） in the Dehong prefecture of Yunnan in 1989. Since then, Yunnan province has had the highest numbers of HIV-1 infections and AIDS-related deaths in China. It is also considered a site for future vaccine studies, but a systematic study of the molecular epidemiology of HIV-1 in this province has not been done. The goal of this study is to conduct a molecular characterization of HIV-1 throughout every region of Yunnan. Using immunoassays, we cumulatively identified and mapped 103,015 cases of HIV-1 infections in Yunnan by 2004. We obtained HIV-1 p17 genes from the serum of 321 patients using an RT-PCR technique. DNA sequences derived from the amplified products were subjected to phylogenetic analyses. These patients were from Yunnan's all sixteen prefectures representing four risk groups, eleven ethnic populations, and ten occupations. The majority of these infections （189/321;58.9%） were found among the Han population. Using phylogenetic analysis we have identified three major genotypes circulating in the province including subtypes C/CRF07/08C （53%）, CRF01E （40.5%）, and B （6.5%）. For subjects with known risk factors, there were two major patient populations: IDUs （51.7%） and sexually transmitted cases （45.9%）. The rest were through vertical transmission and blood transfusion （2.4%）. Among IDUs 90.9% had C/CRF07/08C viruses whereas 85.4% of CRF01AE infections were acquired through sexual transmission. Only 6.8% of HIV infections among IDUs were due to subtype CRF01E. Geographically, C/CRF07/08C was found throughout the province while CRF01AE was largely confined to the western prefectures bordering Myanmar. Lastly, C/CRF07/08C viruses were found consisted of a group of viruses including C, CRF08C, CRF07C and new BC recombinants by characterizing viral RT genes. This is the first report of a comprehensive, province-wide HIV-1 molecular epidemiology study in Yunnan or anywhere else in Chin
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