引言副流感病毒3型属副黏病毒科呼吸道病毒属,是人和动物呼吸道重要病原.其中牛副流感病毒3型(bovine parainfluenza virus type 3,BPIV3)是引起牛呼吸道疾病的主要病原,危害巨大[1,2].2013年底本实验室首次从江苏、安徽多地患呼吸道疾...
引言副流感病毒3型属副黏病毒科呼吸道病毒属,是人和动物呼吸道重要病原.其中牛副流感病毒3型(bovine parainfluenza virus type 3,BPIV3)是引起牛呼吸道疾病的主要病原,危害巨大[1,2].2013年底本实验室首次从江苏、安徽多地患呼吸道疾病的山羊中分离鉴定出山羊PIV3,成为该病毒属的新成员[3].HN、F、M、N是病毒的主要结构蛋白,其中糖蛋白HN具有血凝素和神经氨酸酶活性,介导病毒入胞,也是主要保护性抗原[4].本研究合成JS2013分离株HN基因,构建重组杆状病毒,获得重组蛋白,为病毒HN蛋白的功能研究以及疫苗和检测方法的建立提供有力工具.
[Objective] This study aimed to investigate the genetic variation of g E gene of an epidemic pseudorabies virus（PRV） strain and its pathogenicity to piglets. [Method] By serial passage in Vero cells, a PRV strain wa...
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[Objective] This study aimed to investigate the genetic variation of g E gene of an epidemic pseudorabies virus（PRV） strain and its pathogenicity to piglets. [Method] By serial passage in Vero cells, a PRV strain was isolated from the brain tissues of stillborn fetuses delivered by sows with suspected PRV infection and preliminarily identified by PCR. g E gene of the isolated PRV strain was amplified and sequenced for phylogenetic analysis. In addition, the pathogenicity of the isolated PRV strain to 6-week-old piglets was evaluated. [Result] A PRV strain was successfully isolated and named PRV N5 B strain, which could proliferate in Vero cells and TCID50 of the 15 thgeneration virus liquid reached 10^7.125/0.1 ml. Specific bands could be amplified by PCR. g E gene in the isolated PRV strain was 1 740 bp in length. A phylogenetic tree was constructed based on full-length g E sequences, which showed that PRV N5 B strain and PRV strains isolated since 2012 were clustered into the same independent category and shared 99.7%-100% homology of nucleotide sequences. Compared with related sequences published previously, there were insertions of three consecutive bases at two loci. Animal experiments showed that intranasal inoculation of 6-week-old piglets with 2 ml of PRV N5 B strain（10^6/0.1 ml） led to a mortality rate of 100%. [Conclusion] In this study,genetic variability of g E gene in PRV N5 B isolate and its pathogenicity to piglets were analyzed, which provided a theoretical basis for the development of new vaccines to prevent and control porcine pseudorabies.
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