BALB/c mice were immunized using synthetic tandem polypeptide of Cap protein epitope of porcine circovirus type 2 （PCV2） as the antigen. By using lym-phocyte hybridoma technique, a hybridoma cellline stably secretin...
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BALB/c mice were immunized using synthetic tandem polypeptide of Cap protein epitope of porcine circovirus type 2 （PCV2） as the antigen. By using lym-phocyte hybridoma technique, a hybridoma cellline stably secreting monoclonal an-tibody against PCV2-rCap protein was successful y obtained and named as 670#. The ascites titer of the obtained monoclonal antibody was 1∶100 000. Western blot results showed that the monoclonal antibody could react with prokaryotical y ex-pressed PET32a-ORF2 recombinant protein, eukaryotical y expressed ORF1-ORF2 tandem protein and PCV2 whole virus celllysate. Indirect EILSA demonstrated that the monoclonal antibody could bind with ORF1-ORF2 tandem protein. Indirect im-munofluorescence assay （IFA） indicated that the monoclonal antibody could identify native PCV2 virus. The preparation of this monoclonal antibody provided technical tools for epitope analysis and molecular diagnosis of PCV2 virus.
本文利用地理信息系统(Geographic Information System,GIS)技术-Arc GIS软件实现猪粮比价数据管理和等级风险评估的可视化,并建立猪粮比价评估系统。研究以江苏省宿迁市为例,分析猪粮比价与生猪价格、玉米价格和豆粕价格相关性,通过建...
详细信息
本文利用地理信息系统(Geographic Information System,GIS)技术-Arc GIS软件实现猪粮比价数据管理和等级风险评估的可视化,并建立猪粮比价评估系统。研究以江苏省宿迁市为例,分析猪粮比价与生猪价格、玉米价格和豆粕价格相关性,通过建立GM(1,1)模型,分别对生猪价格、玉米价格和豆粕价格进行趋势预测,采用灰色关联度建立猪粮比价与其影响因素的关系模型,建立猪粮比价预测模型。同时,提出在整体生猪市场不断发展的情况下,特别是规模养猪场猪粮比价变化频度加快、构成要素增加、更新要求提高,需要对于不同地区,不同市场,不同生产方式,进一步具体分析。
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