Background Based on the hypoxic environment,the energy supply of cancer cells and CSC is associated with glucose transporter-1(Glut1) expression and *** studies have found that autophagy may affect cell glucose *** sp...
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Background Based on the hypoxic environment,the energy supply of cancer cells and CSC is associated with glucose transporter-1(Glut1) expression and *** studies have found that autophagy may affect cell glucose *** speculate that Glut1 is correlated with autophagy in the process of energy supply of *** In this work,we investigate the mechanism inhibition of growth and migration of CD 133+CSCs of laryngeal carcinoma Tu212 cells under hypoxic and low-nutrition *** Tu212 cells expressing CD133 were sorted by MACS and the proportion of CD133+Tu212 cells were assessed by flow *** numbers,the proliferation,and the ability of cell migration of Tu212 CD133+cells under hypoxia and low glucose was markedly higher than that of Tu212 CD133-cells under hypoxia and low glucose or control Tu212 CD133+cells(20%O2,25 mM Glucose),while the apoptotic rate of Tu212 CD133+cells under hypoxia and low glucose was significantly ***,the expression of Glut1 mRNA and protein were higher in Tu212 CD133+laryngeal carcinoma cells under hypoxia and low glucose compared to Tu212 CD133-laryngeal carcinoma cells under hypoxia and low glucose and control Tu212 CD133+cells(20%O2,25 mM Glucose).Meanwhile,we found that autophagy-related(ATG) proteins were changed under hypoxia and low ***,these results demonstrated that overexpression of GLUT1 facilitated the growth of Tu212 CD133+laryngeal carcinoma ***1 shRNA could inhibit the expression of Beclin-1,Atg 7,and Atg 5 mRNA and protein,LC3Ⅱ/LC3Ⅰ of Tu212 CD133+cells under hypoxia and low *** Take together,our studies revealed that hypoxia and glucose starvation may increase the cell growth and migration of laryngeal carcinoma CD 133+cancer stem cells.
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