Human embryonic stem cells(hESCs),derived from the inner cell mass of human blastocysts,are considered to be the most pluripotent of all stem *** feeder-dependent and feeder-free culture systems are applied to maintai...
Human embryonic stem cells(hESCs),derived from the inner cell mass of human blastocysts,are considered to be the most pluripotent of all stem *** feeder-dependent and feeder-free culture systems are applied to maintain self-renewal ***,hESCs of different culture systems showed different morphology,there might be different transcriptional and functional differences between these *** test this hypothesis,we first derived three hESCs lines under the culture system of human foreskin fibroblasts (HFF) combined with mTESR1,and the stem cell characteristics were *** then,we conducted a large-scale transcriptional profiling of IncRNAs and mRNAs in these three pairs of hESCs cultured under feeder-dependent and feeder-free culture systems using microarray technology.
暂无评论