为板栗FLC同源基因控制开花的相关功能及春化作用的相关分子机理提供研究基础,以罗田板栗为研究材料,分离并鉴定板栗花芽调控相关基因FLC,依据NCBI数据库提供的EST序列片段,利用RACE技术,从板栗叶片总RNA中分离了与开花相关的基因-CmFLCcDNA的序列,通过在线分析预测其序列同源性及3D结构,以pBluescript SK plus作为过渡载体构建其干扰表达载体。结果表明,该序列全长为968bp,其中有1个编码含227个氨基酸蛋白序列的开放阅读框。CmFLC蛋白的理论等电点为5.80,分子质量为25.62kDa,N端含有M盒保守序列,该蛋白的二级结构大部分由无规则卷曲和α-螺旋构成。CmFLC存在M盒、K盒两个特征性序列区域。CmFLC蛋白与毛白杨FLC蛋白的三维结构存在高度相似性。板栗的FLC蛋白属于植物进化分支,且与山核桃的FLC蛋白同属一支。设计特异引物扩增得到FLC基因的正、反义基因干扰片段,酶切结果显示插入片段大小为320bp,板栗FLC的干扰载体pc1301-ubi-CMFLCRNAi构建成功。结论:板栗中存在FLC同源基因,并可能参与板栗开花相关功能及春化调控。
The growth and development of staminate inflorescence and anatomic structure of male chestnut flower were observed. Results showed that staminate in-florescence on the base of branch formed first, then upward successi...
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The growth and development of staminate inflorescence and anatomic structure of male chestnut flower were observed. Results showed that staminate in-florescence on the base of branch formed first, then upward successively. About 50 days were needed from the formation of staminate inflorescence on the base of branch to ful y develop the staminate inflorescence on the top of the branch. On the same staminate inflorescence, male flower clusters of the base formed first, then upward successively. About 20 days were needed from the formation of stami-nate inflorescence on the base of the male flower cluster to ful y develop the stami-nate inflorescence on the top of the branch. 5-7 male flowers forming a cluster, the flower number in a cluster was odd number usual y, and there was one on the top and each two paral el y arranged downward. The flower on the top came into bloom first, and then downward successively. The flowers paral el y arranged came into bloom at the same time. Sporangium of male flower of chestnut was monolocular. There were a large number of pol en grains in the sporangium. There were large differences between the development process of different sporangium in one male flower. Chestnut had larger quantity of male flowers and pol en and long period of pol ination compared with female flower. It is remained to be further studied whether it was necessary for anemophilous pol ination.
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