转化生长因子-β (Transforming growth factor-beta, TGF-β)作为一种细胞因子,隶属于TGF-β超家族,它在细胞生长和分化过程中担任着关键的调节角色。近年来许多研究表明TGF-β在结直肠癌发生和发展过程中的作用具有两面性,它既有可以促进肿瘤的生长和转移,也有可以在某些特定情况下通过抑制TGF-β的活性来增强结直肠癌的治疗效果。本文旨在综述近年来TGF-β在结直肠癌发生和发展过程中的双重作用及潜在的机制,以期为结直肠癌的治疗提供参考依据。Transforming growth factor-beta (TGF-β), a cytokine belonging to the TGF-β superfamily, plays a key regulatory role in the process of cell growth and differentiation. In recent years, many studies have shown that the role of TGF-β in the occurrence and development of colorectal cancer has two sides, which can promote the growth and metastasis of tumors, and can enhance the therapeutic effect of colorectal cancer by inhibiting the activity of TGF-β in some specific cases. This article aims to review the dual role of TGF-β in the occurrence and development of colorectal cancer in recent years and its potential mechanism, in order to provide a reference for the treatment of colorectal cancer.
目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UVA和H2O2分别处理细胞使其衰老,建立早衰模型。此后进行CPE的慢病毒转染,获得CPE稳定敲除的细胞株。通过qRT-PCR和WB技术,检测CPE和衰老标志物蛋白及mRNA的表达。分别采用cck8和SA-β-gal染色检测CPE敲降后NHSFs的增殖和衰老程度。最后采用qRT-PCR检测CPE敲降后NHSFs促炎症因子的表达水平。结果:N1组CPE蛋白和mRNA表达最高,其次是N2组,N3组最少,早衰细胞模型中CPE蛋白和mRNA的表达减低,CPE敲降后NHSFs蛋白和mRNA的表达均升高、细胞活性降低、细胞衰老程度明显加快和促炎症因子mRNA表达升高。结论:CPE可作为潜在衰老标志物且其高表达可延缓衰老。Objective: To explore the correlation between the expression of CPE and cellular senescence. Methods: Human foreskin fibroblasts (NHSF cells) isolated from healthy human donors (5~20 years old) who underwent surgical defibrillation were selected for passage. Here, we defined “NHSFs of less than 15 generations as group N1, NHSFs of 15~25 generations as group N2, and NHSF of more than 45 generations as group N3”. Then the cells were treated with UVA and H2O2 respectively to make them senescent, and the premature aging model was established. Then lentiviral transfection of CPE was performed to obtain stable CPE knockout cell lines. The expression of CPE and aging marker protein and mRNA were detected by qRT-PCR and WB. The proliferation and senescence of NHSFs after CPE knockdown were detected by cck8 and SA-β-gal staining, respectively. Finally, the expression level of NHSFs proinflammatory factors after CPE knock-down was detected by qRT-PCR. Results: The expressions of CPE protein and mRNA were the highest in N1 group, followed by N2 group and N3 group, and the expressions of CPE protein and mRNA were decreased in the progeria cell model. After CPE knockdown, the expressions of NHSFs protein and mRNA were both increased, the cell activity was decreased, the degree of cell senility was significantly accelerated, and the mRNA expression of proinflammatory factors was increased. Conclusion: CPE can be used as a potential senescence marker and its high expression can delay senescence.
目的:通过实证干预的方法,探索正念认知疗法对医学生焦虑情绪的干预效果;方法:通过SAS焦虑自评量表筛选出40名焦虑分值大于50的医学生,按照随机化原则分成两组,分为实验组和对照组,一组20人。对照组学生不进行任何处理,实验组学生由专业老师指导,进行为期8周的正念认知训练,一周练习1次,每次练习45分钟,有具体的主题和内容。8周练习结束后,再次通过SAS量表对两组被试的焦虑水平进行测量,检验正念认知疗法对医学生焦虑情绪的干预效果如何。结果:实验组学生在进行8周正念认知训练后,实验组的焦虑得分显著下降。结论:正念认知疗法对医学生焦虑情绪的干预效果显著。Purpose: To explore the effect of Mindfulness-Based Cognitive Therapy (MBCT) on the anxiety of medical students through empirical intervention methods. Method: Forty medical students with an anxiety score greater than 50, as measured by the Self-Rating Anxiety Scale (SAS), were randomly divided into two groups: an experimental group and a control group, with 20 students in each group. The control group did not receive any intervention, while the experimental group received an 8-week mindfulness-based cognitive training guided by a professional instructor, with one session per week, each lasting 45 minutes, and having specific themes and content. After the 8-week training, the anxiety levels of both groups were measured again using the SAS to test the effect of MBCT on the anxiety of medical students. Results: The anxiety scores of the experimental group significantly decreased after the 8-week MBCT training. Conclusion: Mindfulness-Based Cognitive Therapy has a significant intervention effect on the anxiety of medical students.
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