High performance liquid chromatography(HPLC) is one of the analytical techniques used most frequently. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this p...
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High performance liquid chromatography(HPLC) is one of the analytical techniques used most frequently. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this paper, the overlapped chromatograms and spectra system of 1-chloro-2,4-dinitrobenzene and 3,5-dinitrobenzoic acid in the presence of 2,4-dinitrobenzoic acid were resolved by using the alternating trilinear decomposition(ATLD) combined with HPLC-DAD. Moreover, both were determined simultaneously, whose recoveries were(102.1±2.2)% and(97.6±3.6)%, respectively. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures and quantifying accurately. It revealed the ATLD algorithm was one of the approaches to solve second-order calibration problem in HPLC-DAD.
In this article, the preparation of core-shell fluorescent dye nanoparticles doped with rhodamine B isothiocyanate by using novel method is reported, which involves synchronous hydrolysis of tetraethoxysilane(TEOS) in...
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In this article, the preparation of core-shell fluorescent dye nanoparticles doped with rhodamine B isothiocyanate by using novel method is reported, which involves synchronous hydrolysis of tetraethoxysilane(TEOS) in water in oil microemulsion with the combination of N-(β-amimoethyl)-γ-amino propyltriethoxy-silane(AEAPS) and rhodamine B isothiocyanate(TRITC). This method conquers the disadvantages of traditional preparation method about organic dye doped core-shell nanoparticles such as the leakage of fluorescent dyes and the low encapsulation efficiency of fluorescent dyes. The fluorescent nanoparticle prepared by using the novel method have the advantages such as high fluorescence and good fluorescent stability, which are hopeful to be applied in the field of biological labelling.
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