A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using ...
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A piezoelectric immunosensor has been developed for the detection of Hepatitis B surface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self assemble monolayers(SAMs). Using a bifunctional cross linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0 2-12 0 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0 2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.
荧光分析法因具有高灵敏度、高选择性、信息量丰富、检测限低等特点,被广泛应用在生物化学、医药化学、环境化学、农业化学、食品科学等研究领域的痕量物质分析.随着荧光技术的发展,已由原来的单点单波长测量逐步发展成为可同时进行激发发射扫描的激发-发射矩阵(excitation-emission matrix or EEM)荧光分析.EEM荧光使用,大大简化了复杂组分的繁琐分离过程,提高了荧光分析的灵敏度、选择性和实用性,还可进行指纹分析和技术鉴定.省力，可以充分利用化学信息，同时提高了荧光分析的灵敏度和选择性。本文论述了化学计量学与EEM荧光技术的结合，不仅是荧光技术的一个重要发展方向，同时也拓展了分析化学的应用领域.显示了化学计量学独特的魅力。
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