In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB *** the operation,the effects of 5-FU concentratio...
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In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB *** the operation,the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33ING1 were measured in *** results were as follows: p33ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of *** MTT results also proved that high expression of p33ING1 mRNA can increase cell’s sensitivity to chemical drug *** detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.
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