Previous research has confirmed that hematopoietic cells and endothelial cells originate in a common mesodermal precursor named as hemangioblast at specific time and locations during embryonic development. Hematopoiet...
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Previous research has confirmed that hematopoietic cells and endothelial cells originate in a common mesodermal precursor named as hemangioblast at specific time and locations during embryonic development. Hematopoietic cells and endothelial cells were originated gradually from hemangioblast by specialization, determination and differentiation step by step. The author reviews the origin, markers, commitment, and differentiation of hemangioblast, and presents some research evidence on the transcriptional factors and influencing genes for the specialization of hemangioblast. Further research on specialization genes of the hemangioblast will be critical for a better understanding of the events involved in hematopoietic and endothelial cell differentiation as well as for utilizing this cell population for clinical applications.
目的:明确维生素E琥珀酸酯(vitamin E succinate,VES)诱导的人口腔鳞癌Tca8113细胞的凋亡作用,初步探讨其凋亡机制。方法:PI单染色和Annexin Ⅴ-PI双染色后,流式细胞术检测VES对细胞周期的影响及细胞凋亡率;RT-PCR、west-ernblot法检测...
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目的:明确维生素E琥珀酸酯(vitamin E succinate,VES)诱导的人口腔鳞癌Tca8113细胞的凋亡作用,初步探讨其凋亡机制。方法:PI单染色和Annexin Ⅴ-PI双染色后,流式细胞术检测VES对细胞周期的影响及细胞凋亡率;RT-PCR、west-ernblot法检测促凋亡基因bax在mRNA水平和蛋白水平上的表达。结果:VES能显著抑制Tca8113细胞的生长,诱导出现典型的凋亡峰,bax基因及蛋白表达上调。结论:VES能诱导口腔鳞癌细胞Tca8113的凋亡,该作用可能与bax基因表达上调有关。
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