Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,...
详细信息
Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,bioactive compounds in traditional Chinese medicines,biomarkers in animal biofluids,toxic components in food or environmental *** this study,we used this platform to comprehensively screen all of the flavonoids in rhizoma smilacis glabrae based a self-made database using high resolution MS and MS/MS of ultra-high performance liquid chromatography-quadrupole-time of flight/mass spectrometry (UHPLC-Q-TOF/MS),we found over thirty potential new flavonoids,further qualified using LC-SPE-NMR,among of them,six compounds were identified as new flavonoids by bath of 1Dand 2D NMR spectra.
In the current study we investigated the pharmacokinetic profile of an anti-arthritic herbalproduct,Qingfu Guanjieshu (QFGJS) capsule,in rats by profiling the primary representativecompounds sinomenine (SI),paeoniflor...
详细信息
In the current study we investigated the pharmacokinetic profile of an anti-arthritic herbalproduct,Qingfu Guanjieshu (QFGJS) capsule,in rats by profiling the primary representativecompounds sinomenine (SI),paeoniflorin (PF) and paeonol (PA) with a validated High Performance Liquid Chromatography–Diode Array Detector (HPLC–DAD) *** chromatographic separation was achieved with an ODS columncomposed of acetonitrile and aqueous phase (containing 0.1％ formicand a mobile phaseacid, adjusted withtriethylamine to pH 3.5±0.2). The developed method was validated with excellent precision,accuracy, recovery and stability in calibration ranges from 0.06 to 11.62μg/ml for SI, from 0.09 to35.70μg/ml for PF, and from 0.15 to 4.53μg/ml for PA (with r2>0.999 for all three compounds).
Neo-tanshionlactone was first isolated from Tanshen,the rhizome of Salvia miltiorrhizaBunge,as an anti-tumor agent with unique structure in *** analysis showed that neo-tanshionlactone expresses selective antibreast c...
详细信息
Neo-tanshionlactone was first isolated from Tanshen,the rhizome of Salvia miltiorrhizaBunge,as an anti-tumor agent with unique structure in *** analysis showed that neo-tanshionlactone expresses selective antibreast cancer activity. Firstly, it was active againstbreast cancer cell line but also showed insignificant activity against other cell lines, like skincancer and prostate cancer, which implies potential targeted therapy against breast ***, it was active against both Estrogen Receptor positive (ER+) breast cancer cell lines(MCF-7 and ZR-75-1) and Human Epidermal Growth Factor Receptor 2 over-expressing breastcancer cell line (SK-BR-3, HER2+), but was inactive against two ER- cell lines (MDA MB-231,and HS 587-T). Compare with the ER antagonist, tamoxifen, nto-tanshionlactone showed 10-foldmore potent and 20-fold more selective against the ER+ cell lines. Because of its potent andunique activity, it was total synthesized and the structure activity relationship (SAR) study furtherconfirmed the selective antibreast cancer activity. Both ih vitro and ih vivo studies were carriedout and supported neo-tanshionlactone and its derivate can be further studied as clinical trialcandidate. However, its mechanism of action is complex since SK-BR-3 is ER- which means ithas distinct mechanism from tamoxifen and other existing antibreast cancer drugs. Clarification ofthe mechanism may provide novel target for breast cancer treatment and is undergoing in ourlaboratory.
暂无评论