Small RNAs主要包含miRNA、piRNA、siRNA、snoRNA等RNA分子,它们参与基因表达与调控、RNA的加工与剪切、蛋白质翻译、遗传"入侵"抑制、配子发生等重要生物学过程,是生命活动中必不可少的调控因子.Small RNA中最主要的组成是mi...
Small RNAs主要包含miRNA、piRNA、siRNA、snoRNA等RNA分子,它们参与基因表达与调控、RNA的加工与剪切、蛋白质翻译、遗传"入侵"抑制、配子发生等重要生物学过程,是生命活动中必不可少的调控因子.Small RNA中最主要的组成是microRNA,可通过识别靶mRNA实现转录后的基因调控.研究表明癌症、心血管疾病、免疫系统疾病等多种疾病均与miRNA的调控作用密切相关.
Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,...
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Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,bioactive compounds in traditional Chinese medicines,biomarkers in animal biofluids,toxic components in food or environmental *** this study,we used this platform to comprehensively screen all of the flavonoids in rhizoma smilacis glabrae based a self-made database using high resolution MS and MS/MS of ultra-high performance liquid chromatography-quadrupole-time of flight/mass spectrometry (UHPLC-Q-TOF/MS),we found over thirty potential new flavonoids,further qualified using LC-SPE-NMR,among of them,six compounds were identified as new flavonoids by bath of 1Dand 2D NMR spectra.
In the current study we investigated the pharmacokinetic profile of an anti-arthritic herbalproduct,Qingfu Guanjieshu (QFGJS) capsule,in rats by profiling the primary representativecompounds sinomenine (SI),paeoniflor...
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In the current study we investigated the pharmacokinetic profile of an anti-arthritic herbalproduct,Qingfu Guanjieshu (QFGJS) capsule,in rats by profiling the primary representativecompounds sinomenine (SI),paeoniflorin (PF) and paeonol (PA) with a validated High Performance Liquid Chromatography–Diode Array Detector (HPLC–DAD) *** chromatographic separation was achieved with an ODS columncomposed of acetonitrile and aqueous phase (containing 0.1％ formicand a mobile phaseacid, adjusted withtriethylamine to pH 3.5±0.2). The developed method was validated with excellent precision,accuracy, recovery and stability in calibration ranges from 0.06 to 11.62μg/ml for SI, from 0.09 to35.70μg/ml for PF, and from 0.15 to 4.53μg/ml for PA (with r2>0.999 for all three compounds).
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