Small RNAs主要包含miRNA、piRNA、siRNA、snoRNA等RNA分子,它们参与基因表达与调控、RNA的加工与剪切、蛋白质翻译、遗传"入侵"抑制、配子发生等重要生物学过程,是生命活动中必不可少的调控因子.Small RNA中最主要的组成是mi...
Small RNAs主要包含miRNA、piRNA、siRNA、snoRNA等RNA分子,它们参与基因表达与调控、RNA的加工与剪切、蛋白质翻译、遗传"入侵"抑制、配子发生等重要生物学过程,是生命活动中必不可少的调控因子.Small RNA中最主要的组成是microRNA,可通过识别靶mRNA实现转录后的基因调控.研究表明癌症、心血管疾病、免疫系统疾病等多种疾病均与miRNA的调控作用密切相关.
Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,...
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Integrated liquid chromatography-solid phase extraction-nuclear magnetic resonance/mass spectrometry (LC-SPE-NMR/MS) is powerful platform,which was used to analyze the target components in complex system,for instance,bioactive compounds in traditional Chinese medicines,biomarkers in animal biofluids,toxic components in food or environmental *** this study,we used this platform to comprehensively screen all of the flavonoids in rhizoma smilacis glabrae based a self-made database using high resolution MS and MS/MS of ultra-high performance liquid chromatography-quadrupole-time of flight/mass spectrometry (UHPLC-Q-TOF/MS),we found over thirty potential new flavonoids,further qualified using LC-SPE-NMR,among of them,six compounds were identified as new flavonoids by bath of 1Dand 2D NMR spectra.
Neo-tanshionlactone was first isolated from Tanshen,the rhizome of Salvia miltiorrhizaBunge,as an anti-tumor agent with unique structure in *** analysis showed that neo-tanshionlactone expresses selective antibreast c...
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Neo-tanshionlactone was first isolated from Tanshen,the rhizome of Salvia miltiorrhizaBunge,as an anti-tumor agent with unique structure in *** analysis showed that neo-tanshionlactone expresses selective antibreast cancer activity. Firstly, it was active againstbreast cancer cell line but also showed insignificant activity against other cell lines, like skincancer and prostate cancer, which implies potential targeted therapy against breast ***, it was active against both Estrogen Receptor positive (ER+) breast cancer cell lines(MCF-7 and ZR-75-1) and Human Epidermal Growth Factor Receptor 2 over-expressing breastcancer cell line (SK-BR-3, HER2+), but was inactive against two ER- cell lines (MDA MB-231,and HS 587-T). Compare with the ER antagonist, tamoxifen, nto-tanshionlactone showed 10-foldmore potent and 20-fold more selective against the ER+ cell lines. Because of its potent andunique activity, it was total synthesized and the structure activity relationship (SAR) study furtherconfirmed the selective antibreast cancer activity. Both ih vitro and ih vivo studies were carriedout and supported neo-tanshionlactone and its derivate can be further studied as clinical trialcandidate. However, its mechanism of action is complex since SK-BR-3 is ER- which means ithas distinct mechanism from tamoxifen and other existing antibreast cancer drugs. Clarification ofthe mechanism may provide novel target for breast cancer treatment and is undergoing in ourlaboratory.
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