以传统水田种植(conventional tillage,CT)为对照,对水上种植(culture in water-CW)水稻的光合特性、根系特点和部分农艺性状进行比较研究。结果发现,水稻在CW的光合速率(PN)和气孔导度(gs)增加幅度较大,根条数、根系活力和酶活性显著提...
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以传统水田种植(conventional tillage,CT)为对照,对水上种植(culture in water-CW)水稻的光合特性、根系特点和部分农艺性状进行比较研究。结果发现,水稻在CW的光合速率(PN)和气孔导度(gs)增加幅度较大,根条数、根系活力和酶活性显著提高,分蘖数和有效穗数分别增加17.91%和24.07%;水分利用率、株高、结实率和千粒质量却无明显差异。结果表明,CW通过提高根系吸收能力、增加分蘖数和有效穗数及提高光合速率,达到增产的目的,是值得推广的种植方式。
ETHNOPHARMACOLOGICAL RELEVANCE:A medicinal herb Crotalaria Sessiliflora has been used to treat various diseases including diuretic,cardiotonic,analgesic,and anticancer in folk traditional medicine in Southern of *** a...
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ETHNOPHARMACOLOGICAL RELEVANCE:A medicinal herb Crotalaria Sessiliflora has been used to treat various diseases including diuretic,cardiotonic,analgesic,and anticancer in folk traditional medicine in Southern of *** aim of the present study was to investigate the anticancer activity of Crotalaria Sessiliflora in pancreatic cancer *** AND METHODS:The cytotoxic and apoptotic effect of alkaloids-rich fraction of Crotalaria sessiliflora(ACS) on human pancreatic cancer cells 13.6pl and Bxpc-3 cells were evaluated using MTT and TUNEL assays,and Annexin V/Propidium iodide detection by flow *** effect of ACS on cell cycle was analyzed by flow cytometry with propidium *** apoptotic and cell cycle effects were also evaluated by RT-PCR and western blot ***:Alkaloids-rich fraction of Crotalaria sessiliflora repressed clonogenecity and proliferation,induced apoptosis,and enhanced accumulation in the G2 phase of pancreatic cancer *** addition,a second population of cells expressing cyclin Bl had developed polyploidy and DNA content consistent with 8 N *** with adriamycin,a known inducer of p53,ACS-treated pancreatic cancer cells expressed p53 only at high concentrations and p53 expression was not coordinated with G2 arrest or ***,ACS suppressed the transcription of genes associated with cell cycle regulation,anti-apoptosis,and ***:These data showed that ACS blocks proliferation and induces apoptosis in human pancreatic cancer cells through G2 arrest in association with persistent cyclin Bl expression,failure to completely activate cdc2,and continued DNA synthesis through a pathway that is unrelated to altered expression of *** can be used as an anticancer drug for pancreatic carcinoma patients.
Pancreatic cancer stem cells（PCSC） are a heterogeneous population with unique cellular phenotypes and tumorigenic *** expansion of PCSC is the major pathogenic factor contributing to cancer initiation,drug resistanc...
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Pancreatic cancer stem cells（PCSC） are a heterogeneous population with unique cellular phenotypes and tumorigenic *** expansion of PCSC is the major pathogenic factor contributing to cancer initiation,drug resistance,and *** have previously demonstrated that PCSC selectively expressing CD24,CD44,and ESA surface markers(pCSC)processes self-renewal capability with sustained expression of the RON receptor tyrosine ***-directed delivery of chemotherapeutic agents using RON as the targeting moiety could show therapeutic effect on ***,a significant number of PCSC are resistant to the cytotoxic effect elicited by ***,novel strategy that is effective in killing pcsc is urgently needed to overcome CSC drug resistance and to show therapeutic *** pCSC derived from pancreatic cancer L3.6pl as the model,we demonstrated in vitro that the combined inhibition of RON and mTOR pathways are highly effectively in killing and eliminating PCSC+24/44/*** therapeutic effectiveness was achieved by using small molecule inhibitors BMS-777607（highly specific RON with ICso of 1.3 nM） and AZD8055（a specific and ATP-competitive mTOR inhibitor with Ic50 of 0.8 nM）,*** of L3.6pl cells with BMS-77067 caused moderate cell growth inhibition and induced extensive polyploidy with multiple chromosome *** cycle analysis revealed that although affected cells were blocked at pro-metaphase and failed to undergo *** cells were alive and can antagonize chemoagent-induced cytotoxic ***,pCSC were even more resistant to BMS-777607-induced growth inhibition and polyploid *** observations promoted us to screening signaling pathways that are important for CSC *** eight pathways analyzed using individual specific inhibitors,we identified mTOR as a molecule essential for CSC cell *** of mTOR activities by AZD8055 in combination with BMS-777607 not only
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