Reactive oxygen species(ROS)are crucial in the pathogenesis of diabetic nephropathy(DN).X-box binding protein 1(XBP1),a key mediator of endoplasmic reticulum(ER)stress,has been proved having the capability of preventi...
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Reactive oxygen species(ROS)are crucial in the pathogenesis of diabetic nephropathy(DN).X-box binding protein 1(XBP1),a key mediator of endoplasmic reticulum(ER)stress,has been proved having the capability of preventing oxidative *** this study,the effects of spliced XBP1(XBP1S)on high glucose(HG,30 mmol/L D-glucose)induced ROS production and apoptosis were investigated in cultured renal mesangial cells(MCs).ROS level was detected by dihydroethidium(DHE)fluorescent probe *** blot were used to evaluate the XBP1S,pro-caspase3,cleaved-caspase3,Bax and Bcl2 protein *** cytometry was used to detect cell apoptosis and *** of recombinant adenovirus vector carrying XBP1S gene(Ad-XBP1S)was used to upregulate XBP1S *** results showed that HG treatment significantly reduced XBP1S protein levels in the cultured *** Cytometry results showed that the Annexin-V positive cell was increased significantly after HG *** of Ad-XBP1S reversed HG-induced ROS ***-XBP1S trasnfection suppressed the HG-induced increases in both total caspase 3 and cleaved caspase 3;Similarly,the Ad-XBP1S trasnfection reversed the HG-induced increase in the ratio of Bax/*** blot results demonstrated that Diphenylene-chloride iodonium(DPI,10-6 mol/L),an inhibitor of NADPH oxidase,inhibited HG induced Bax/Bcl2,pro-caspase3 and cleaved-caspase3 protein *** results suggested that suppression in XBP1S pathway of ER stress was involved in HG-induced *** might be a downstream mediator of XBP1S in regulating cell apoptosis.
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