目的:研究紫术消瘤散对宫颈高危型人乳头瘤病毒(HR-HPV)持续感染湿热瘀结型患者阴道菌群的调控作用,探讨紫术消瘤散清除人乳头瘤病毒的作用机制。方法:通过随机对照的临床试验研究,选取来源于福建省第二人民医院妇科门诊及体检中心,符合间隔12个月或以上,连续两次同一亚型HR-HPV检测为阳性者,且严格遵循三阶梯(HPV + TCT–阴道镜检查–宫颈活检)的诊疗规范,经由宫颈活检病理明确诊断为 ≤ LSIL,中医辨证为湿热瘀结型的患者64例,按随机数字表法分为治疗组和对照组。每组32例。治疗组:紫术消瘤散宫颈局部上药,每3天1次,1次4 g,1个月1个疗程,连续使用3个疗程。对照组:予阴道用乳杆菌活菌胶囊,每晚睡前清洗外阴后塞入阴道深部,10天为1个疗程,连续用药3个疗程。观察两组治疗前后患者HPV转阴率、中医证候积分、阴道微生态指标(菌群密集度、菌群多样性、优势菌、pH、Nugent评分)的变化。结果:① 治疗组HR-HPV转阴率(65.6%)比对照组(37.5%)高,差异有统计学意义(P P P Objective: To study the regulatory effect of Zishu Xiaoliu Powder on vaginal flora in patients with persistent cervical high-risk human papillomavirus (HR-HPV) infection of damp-heat stasis type, and to explore the mechanism of action of Zishu Xiaoliu Powder in clearing human papillomavirus. Methods: Through a randomized controlled clinical trial, patients from the gynecological outpatient clinic and physical examination center of the Second People’s Hospital of Fujian Province who were positive for the same subtype of HR-HPV for two consecutive times with an interval of 12 months or more and strictly followed the three-step (HPV + TCT-colposcopy-cervical biopsy) diagnosis and treatment standards were selected. 64 patients were diagnosed as ≤LSIL by cervical biopsy pathology and damp-heat stasis type by TCM syndrome differentiation. They were divided into treatment group and control group according to the random number table method. 32 cases in each group. Treatment group: Zishu Xiaoliu Powder was applied topically to the cervix, once every 3 days, 4g each time, 1 month as a course of treatment, and 3 courses of treatment were used continuously. Control group: Lactobacillus capsules were used for vaginal use, and the capsules were inserted deep into the vagina after cleaning the vulva every night before going to bed. 10 days was a course of treatment, and 3 courses of treatment were used continuously. The changes in the HPV negative conversion rate, TCM syndrome scores, and vaginal microecological indicators (bacterial d
本研究旨在深度剖析长链非编码RNA P53和P21在大鼠动脉粥样硬化模型中的作用及应用潜能。择取60只大鼠,随机平均划分为正常组与粥样硬化组,借由构建大鼠动脉粥样硬化模型,综合运用多种实验技术(如免疫组织化学染色、实时荧光定量PCR、蛋白质印迹法、酶联免疫分析等)及仪器(如彩色多普勒超声诊断仪),对两组中P53和P21的表达状况、调控机制及其与疾病演进的关联予以系统性且全方位的解析。研究成果有望为动脉粥样硬化的诊断与治疗提供新颖且极具价值的靶点与策略。This study aims to deeply analyze the role and application potential of long non-coding RNA P53 and P21 in rat atherosclerosis model. 60 rats were selected and randomly divided into normal group and atherosclerosis group. By constructing rat atherosclerosis model, a variety of experimental techniques (such as immunohistochemistry staining, real-time fluorescence quantitative PCR, protein blotting, enzyme-linked immunosorbent assay, etc.) and instruments (such as color Doppler ultrasound diagnostic instrument) were used to systematically and comprehensively analyze the expression status, regulatory mechanism and relationship between P53 and P21 and disease progression in the two groups. The research results are expected to provide novel and valuable targets and strategies for the diagnosis and treatment of atherosclerosis.
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