SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to ampli...
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SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce poly-morphic DNA fragments. As a result, a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs. By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis, the genetic similarity （SM） coefficient be- tween the 48 accessions was calculated and ranged from 0.58 to 0.98. All the 48 accessions were distinguished from each other. The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645. The results showed that the SRAP markers could be effectively used for analysis of genetic di- versify of JUNCAO germplasms. Additionally, the results also showed that there ex- ist abundant JUNCAO germptasm in the genus Arundo in China. This study provides a new technique for studying the genetic diversify of JUNCAO germplasms. The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future. Moreover, the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.
在籼稻品种R401辐射诱变的M2群体中筛选到一个叶片表皮光滑突变体,在正常条件下,突变体叶片和颖壳光滑无毛。以毛叶粳稻品种Nipponbare和"光身"突变体作为亲本,构建了一个F2群体,通过调查该群体在正常种植条件下的表现,发现Nipponbare和"光身"突变体控制表皮毛性状的差异受单个主基因控制且"光身"为隐性,将该基因暂时命名为GLR3。利用该F2群体,采用BSA法将GLR3定位在第6染色体上,进一步对F2群体中417个典型的叶片光滑单株进行分子标记分析,将该基因定位在In Del标记ID27101和ID27199之间,与两标记相距皆为0.1 c M,两标记的物理位置相距98 kb。
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