Biochar amendment has been extensively used to improve soil quality, improve plant performance and suppress disease in monoculture system, however less studies focused on the underlying mechanisms of the replanting di...
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Biochar amendment has been extensively used to improve soil quality, improve plant performance and suppress disease in monoculture system, however less studies focused on the underlying mechanisms of the replanting disease control. We assessed the effects of applied biochar on Radix pseudostellariae plant growth, rhizosphere soil microbial communities, and the physiological properties of microorganism in consecutively monoculture system. We found biochar addition had little impact on plant physiological parameters, but biochar significantly mediated microbial abundances in the rhizosphere soil of differently consecutive monoculture years, especially decreased the abundances of pathogenic Fusarium oxysporum, Talaromyces helicus and Kosakonia sacchari. Furthermore, biochar amendment has negative effects on the growth of beneficial bacteria, such as Burkholderia ambifaria, Pseudomonas chlororaphis and Bacillus pumilus. Analysis based on the metabolites and metabolome indicated that biochar significantly affect the metabolism process of F. oxysporum, and then inhibit the mycelium growth and abate the virulence on plants. This study explains the mechanisms behind the biochar effect by changing the abundances and metabolism of rhizosphere bacteria and fungi, decreasing the contents of pathogens, and hence improving the environmental conditions for plants growth.
[Background] Rice allelopathy is a natural weed control method that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode...
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[Background] Rice allelopathy is a natural weed control method that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode transcription factors. Our study addresses the MYB transcriptional factor OsMYB57, which modulates rice allelopathic potential;[Materials and methods] The rice accession Kitake(Oryza sativa L. subsp. japonica) and its transgenic lines with increased OsMYB57 expression via a transactivator containing four copies of Virion protein 16(4*VP16, VP64), allelopathic rice accession PI312777(Oryza sativa L. subsp. japonica) and non-allelopathic rice accession Lemont(Oryza sativa L. subsp. indica) were used in this study. ChIP-seq was conducted to identify the motif of relevant genes regulated by OsMYB57 to investigate the regulation network of OsMYB57 and its correlation with rice allelopathy;[Results] Increasing OsMYB57 expression in rice by using the transcription activator VP64 resulted in increased inhibitory ratios against barnyardgrass, while the gene expression levels of OsPAL, OsC4 H, OsOMT, and OsCAD from the phenylpropanoid pathway were also upregulated, and the content of L-phenylalanine increased. Microbe with allelopathic potential, including Penicillium spp. and Bacillus spp., were assembled in the rhizosphere of rice with increased expression of OsMYB57 and responsible for phenolic acid induction. In addition, chromatin immunoprecipitation incorporated with HiSeq demonstrated that OsMYB57 transcriptionally regulated a mitogen-activated protein kinase(OsMAPK11);moreover, OsMAPK11 interacted with OsPAL2;3. The transcriptional ability of OsPAL2;3 was higher in the allelopathic rice PI312777 than in the non-allelopathic rice Lemont, and OsPAL2;3 was negatively regulated by WHIRLY transcriptional factors;[Conclusion] This study reveals that OsMYB57 positively regulates OsMAPK11 and that OsMAPK11 interacts with OsPAL2;3 to modulate rice allelopathy. Ad
The root exudates of Radix pseudostellariae,working as a chemical language between plants and microbes,can deteriorate the microbial community in the rhizosphere in a consecutive monoculture *** assessed the effects o...
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The root exudates of Radix pseudostellariae,working as a chemical language between plants and microbes,can deteriorate the microbial community in the rhizosphere in a consecutive monoculture *** assessed the effects of artificially applied *** root exudates on *** seedling growth,rhizosphere soil microbial communities,and soil physicochemical *** found the effect of phenolic acids and organic acids acted as a driver of changes to the microbial *** throughput sequencing and qRT-PCR analysis demonstrated that the treatment of phenolic acids significantly decreased the relative abundance of Trichoderma,Penicillium,Pseudomonadales,Xanthomonadales,and *** organic acids had a significant negative effect on the relative abundance of Pseudomonadales and Streptomycetales,which led to significantly increased abundance of Fusarium,Xanthomonadales,Micrococcales,and Gemmatim *** based on the non-invasive micro-test techniqueindicated that the root exudates increased the H+ efflux and plasma membrane H+-ATPase activity in the pathogenic fungi and decreased them in the beneficial *** created an acidic environment for the inhibition of beneficial bacteria and accumulation of specialized plant *** study explains the mechanisms behind microflora shift and structure disorder caused by root exudates in a continuously *** rhizosphere soil through chemical language.
Biochar amendment has been extensively used to improve soil quality,improve plant performance and suppress disease in monoculture system,however less studies focused on the underlying mechanisms of the replanting dise...
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Biochar amendment has been extensively used to improve soil quality,improve plant performance and suppress disease in monoculture system,however less studies focused on the underlying mechanisms of the replanting disease *** assessed the effects of applied biochar on Radix pseudostellariae plant growth,rhizosphere soil microbial communities,and the physiological properties of microorganism in consecutively monoculture *** found biochar addition had little impact on plant physiological parameters,but biochar significantly mediated microbial abundances in the rhizosphere soil of differently consecutive monoculture years,especially decreased the abundances of pathogenic Fusarium oxysporum,Talaromyces helicus and Kosakonia ***,biochar amendment has negative effects on the growth of beneficial bacteria,such as Burkholderia ambifaria,Pseudomonas chlororaphis and Bacillus *** based on the metabolites and metabolome indicated that biochar significantly affect the metabolism process of ***,and then inhibit the mycelium growth and abate the virulence on *** study explains the mechanisms behind the biochar effect by changing the abundances and metabolism of rhizosphere bacteria and fungi,decreasing the contents of pathogens,and hence improving the environmental conditions for plants growth.
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