Acute myeloid leukemia(AML) is a heterogeneous disease characterized by complex molecular and cytogenetic *** approaches to predict the prognosis of AML have increasingly attracted *** were 98 non-M3 AML cases and 48 ...
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Acute myeloid leukemia(AML) is a heterogeneous disease characterized by complex molecular and cytogenetic *** approaches to predict the prognosis of AML have increasingly attracted *** were 98 non-M3 AML cases and 48 healthy controls were enrolled in the current *** routine assays for cytogenetic and molecular genetic analyses were performed on the bone marrow samples of AML ***,metabolic profiling of these AML subjects were also performed on the serum samples by combining Ag nanoparticles-based surface-enhanced Raman spectroscopy(SERS) with proton nuclear magnetic resonance(NMR) *** most of the routine biochemical test showed no significant differences between the M0～M2 and M5 groups,the metabolic profiles were significantly different either between AML subtypes or between prognostic risk *** SERS bands were screened to serve as potential markers for AML *** results demonstrated that the classification models for M0～M2 and M5 shared two bands(i.e.,1328 and 741 cm-1),all came from nucleic acid ***,Metabolic profiles provided various differential metabolites responsible for different AML subtypes,and we found altered pathways mainly included energy metabolism like glycolysis,pyruvate metabolism,and metabolisms of nucleic acid bases as well as specific amino acid *** is concluded that integration of SERS and NMR provides the rational and could be reliable to reveal AML differentiation,and meanwhile lay the basis for experimental and clinical practice to monitor disease progression and prognostic evaluation.
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