[Background] Rice allelopathy is a natural weed control method that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode...
详细信息
[Background] Rice allelopathy is a natural weed control method that is regarded as an eco-friendly practice in agroecology. The allelopathic potential of rice is regulated by various genes, including those that encode transcription factors. Our study addresses the MYB transcriptional factor OsMYB57, which modulates rice allelopathic potential;[Materials and methods] The rice accession Kitake(Oryza sativa L. subsp. japonica) and its transgenic lines with increased OsMYB57 expression via a transactivator containing four copies of Virion protein 16(4*VP16, VP64), allelopathic rice accession PI312777(Oryza sativa L. subsp. japonica) and non-allelopathic rice accession Lemont(Oryza sativa L. subsp. indica) were used in this study. ChIP-seq was conducted to identify the motif of relevant genes regulated by OsMYB57 to investigate the regulation network of OsMYB57 and its correlation with rice allelopathy;[Results] Increasing OsMYB57 expression in rice by using the transcription activator VP64 resulted in increased inhibitory ratios against barnyardgrass, while the gene expression levels of OsPAL, OsC4 H, OsOMT, and OsCAD from the phenylpropanoid pathway were also upregulated, and the content of L-phenylalanine increased. Microbe with allelopathic potential, including Penicillium spp. and Bacillus spp., were assembled in the rhizosphere of rice with increased expression of OsMYB57 and responsible for phenolic acid induction. In addition, chromatin immunoprecipitation incorporated with HiSeq demonstrated that OsMYB57 transcriptionally regulated a mitogen-activated protein kinase(OsMAPK11);moreover, OsMAPK11 interacted with OsPAL2;3. The transcriptional ability of OsPAL2;3 was higher in the allelopathic rice PI312777 than in the non-allelopathic rice Lemont, and OsPAL2;3 was negatively regulated by WHIRLY transcriptional factors;[Conclusion] This study reveals that OsMYB57 positively regulates OsMAPK11 and that OsMAPK11 interacts with OsPAL2;3 to modulate rice allelopathy. Ad
暂无评论