Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics *** this pap...
详细信息
Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics *** this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential *** UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 *** the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker *** the projection of positive mode,DM/DN patients and the controls were obviously divided into three ***,two potential markers were identified as phytosphingosine and sphinganine,*** and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid metabolism.
暂无评论