黄曲霉Aspergillusflavus是机会性的动物和植物致病性丝状真菌,保守的PAK(p21-activated protein kinases)样蛋白激酶对信号传导、细胞周期进程和细胞形态发生具有重要作用。通过同源重组方法构建敲除突变株(ΔAflcla4),研究Aflcla4基...
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黄曲霉Aspergillusflavus是机会性的动物和植物致病性丝状真菌,保守的PAK(p21-activated protein kinases)样蛋白激酶对信号传导、细胞周期进程和细胞形态发生具有重要作用。通过同源重组方法构建敲除突变株(ΔAflcla4),研究Aflcla4基因对黄曲霉营养生长、分生孢子产生、菌核形成、黄曲霉毒素合成、耐受性和致病性等方面的影响。与野生型菌株相比,ΔAflcla4菌株菌丝生长速度显著降低,出现无规则分叉,分生孢子产量及色素含量明显减少;细胞壁胁迫及渗透胁迫条件下ΔAflcla4菌株的敏感性增加;突变体对花生及玉米种子的侵染能力下降,产毒能力也显著降低;突变体无法形成菌核,以应对外界不利环境变化。结果表明,黄曲霉PAK样激酶Cla4通过调节相关基因的表达进而影响生长发育、黄曲霉毒素合成及致病性,在黄曲霉中发挥重要的生物学功能。
Aspergillus flavus infects various crops with aflatoxins,and leads to aspergillosis *** is important to discover the elusive regulatory mechanism underlying the virulence of *** study revealed that the Set2 histone me...
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Aspergillus flavus infects various crops with aflatoxins,and leads to aspergillosis *** is important to discover the elusive regulatory mechanism underlying the virulence of *** study revealed that the Set2 histone methyltransferase family,AshA and SetB,involves in morphogenesis and mycotoxin *** ChIP-seq analysis found that Set2 histone methyltransferase regulates fungal development and aflatoxin biosynthesis by regulating related transcriptional factors,including the chromatin region of the sntB,an important transcriptional ***,sntB gene deletion（ΔsntB）,complementary（Com-sntB）,and HA tag fused to sntB（sntB-HA） strains were constructed by using the homologous recombination method,*** results revealed that deletion of sntB inhibited the processes of mycelia growth,conidial production,sclerotia formation,aflatoxin synthesis,and ability to colonize host compared to wild type（WT）,and the defective phenotype of knockout strain ΔsntB could be restored in its complementary strain *** immunoprecipitation sequencing（ChIP-seq） of sntB-HA and WT and RNA sequencing（RNA-seq） of ΔsntB and WT strains revealed that SntB played key roles in oxidative stress response of *** function of catC（encode a catalase） gene was further analyzed based on the integration results of ChIP-seq and *** ?sntB strain,the relative expression level of catC was significantly higher than in WT strain,while a secretory lipase encoding gene（G4B8408359） was *** the stress of oxidant menadione sodium bisulfite（MSB）,the deletion of sntB obvious down-regulated the expression level of *** deletion of catC gene,the mycelia growth,conidial production,and sclerotia formation were inhibited,while ROS level and aflatoxin production were increased compared to the WT *** also showed that the inhibition rate of MSB to ΔcatC strain was significantly lower than that of WT group and AFB1 yield
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