In order to study the pathogenesis of human papilloma virus type 11 (HPV11) and seek for a therapeutic approach of the disease caused by HPV11,we amplified the HPV11/E2 of 644bp length by PCR with HPV11 plasmid DNA,pG...
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In order to study the pathogenesis of human papilloma virus type 11 (HPV11) and seek for a therapeutic approach of the disease caused by HPV11,we amplified the HPV11/E2 of 644bp length by PCR with HPV11 plasmid DNA,pGEM T Easy was used as vector and a clone pTV 644 was *** sequencing of inserted DNA was carried out after selecting and identification,according to the hammerhead structure described by Symon’s. We used computer to analyze the possible secondary cleavage sites on HPV11/E2 mRNA and to predict the secondary structure of substrate and ribozyme to exclude the analogous sequence of substrate combined with ribozyme found in the *** hammerhead ribozyme of RZ3281 against HPV11/E2 mRNA were selected to carry out cleavage reaction in vitro. Results of the experiment showed that 644bp substrate derived from HPV11/E2 can be cleaved site specifically by ribozyme in vitro,cleavage activity showed over 85% under the optimum reaction *** cleavage hammerhead ribozyme can remove effects of gene joint transcript from the vector, that promise the consistent efficiency of ribozyme in vitro and in vivo as far as *** of the experiment demonstrated that the ribozyme will become a highly effective and specific therapy against HPV11 infection.
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