In order to explore the functions of retinol binding protein (RBP4) in regulation of gene expression, yeast two hybrid assay and transient co-transforming were used to detect the interactions between RBP4 and nuclear ...
详细信息
In order to explore the functions of retinol binding protein (RBP4) in regulation of gene expression, yeast two hybrid assay and transient co-transforming were used to detect the interactions between RBP4 and nuclear receptors and the effects of over-expressed RBP4 on trans-activation functions of human estrogen receptor related receptor 1 (hERR1) and human estrogen (hER). The requirement of activation function domain-2 (AF-2) for hER to interact with RBP4 was also detected by the yeast two hybrid assay. The results show that RBP4 could interact with many nuclear receptors including mouse estrogen receptor related receptor 3 (mERR3), retinoid X receptor (RXR), glucocorticoid receptor (GR), progestin receptor (PR), and androgen receptor (AR) in yeast cells. Over-expressed RBP4 could strongly enhance the trans-activation functions of hERR1 and hER in a dose-dependent manner, respectively. RBP4 could also interact with hER in an AF-2-dependent manner.
先天性主动脉缩窄（aortic coarctation ，AC）是较为常见的一种先天性主动脉畸形。在降主动脉上段邻近动脉导管处出现先天性狭窄，并在狭窄的近侧与远侧端产生明显的压力阶差，其发病率约占先天性心脏病的7％～14％［1］。婴幼儿的AC部位多位于动脉导管的近侧端和左锁骨下动脉的远侧段，常伴有动脉导管未闭及缩窄远近段的侧支循环形成［2］。一般当上下肢血压的压力差大于或等于20 mm Hg时就需要积极治疗，且患有AC的婴儿约10％在生后1～6个月内即可发生顽固性心功能衰竭，所以，及时手术常能挽救患儿生命［3］。主动脉缩窄矫治术是治疗主动脉缩窄必需的手术方法。
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