Objective:This study examined whether adenovirus huCT-1 gene transfer promote rubrospinal axons regeneration and recovery of forelimb function after spinal cord injury in adult ***:Gel foam saturated with AdCMV-huCT1 ...
Objective:This study examined whether adenovirus huCT-1 gene transfer promote rubrospinal axons regeneration and recovery of forelimb function after spinal cord injury in adult ***:Gel foam saturated with AdCMV-huCT1 was left into a C3~ 4 lateral funiculus hemisection cavity that completely interrupted one RST.1,4,8 weeks after lesion,RST regeneration were demonstrated with retrograde and anterograde tracing techniques,and function recovery was examined forelimb use asymmetries.
目的探讨肺组织中IL-6含量及mRNA表达在老年大鼠多器官功能障碍综合征(Multiple organ dysfunctionsyndrome,MODS)中的变化。方法80只SD大鼠分为老年组(20月龄)和青年组(3月龄)各40只,建立油酸/脂多糖(OA/LPS)二次致伤老年MODS和青年MOD...
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目的探讨肺组织中IL-6含量及mRNA表达在老年大鼠多器官功能障碍综合征(Multiple organ dysfunctionsyndrome,MODS)中的变化。方法80只SD大鼠分为老年组(20月龄)和青年组(3月龄)各40只,建立油酸/脂多糖(OA/LPS)二次致伤老年MODS和青年MODS模型。观察对照组及伤后2、6、24 h,重要器官(肺、心、肝、肾)病理及功能的变化,通过ELISA方法检测上述组织中IL-6含量,IL-6 mRNA表达用RT-PCR法检测。结果OA/LPS二次致伤MODS模型,肺脏是损害出现最早也是最重的器官,致伤后2 h青年组和老年组PaO2即下降至最低值,伤后6 h,心、肝、肾生化指标达峰值,在相同时相点老年鼠脏器损害重于青年鼠。致伤2h后肺、心、肝和肾组织中IL-6 mRNA表达及蛋白含量均升高,且持续高于正常对照组,以肺组织中升高幅度最大,老年组高于同时相点青年组。结论老年MODS过程中肺损伤出现最早且严重,其原因可能与OA/LPS致伤早期老年鼠肺组织中IL-6 mRNA表达及蛋白含量迅速升高有关。
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