Background & Aims:H19 is one of the earliest identified and studied long noncoding *** is presumed that H19 is essential for regulating development and disease conditions,and is associated with ca
Background & Aims:H19 is one of the earliest identified and studied long noncoding *** is presumed that H19 is essential for regulating development and disease conditions,and is associated with ca
Background & Aims:The liver is frequently subject to insult because of viral infection,alcohol abuse,or toxic chemical *** research has been conducted to identify blood markers that can better discern liver damage...
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Background & Aims:The liver is frequently subject to insult because of viral infection,alcohol abuse,or toxic chemical *** research has been conducted to identify blood markers that can better discern liver damage,but little progress has been achieved in clinical ***,circulating microRNAs(miRNAs) have been reported as potential biomarkers for the noninvasive diagnosis of *** & Results:In this study,we investigated whether plasma miRNAs have diagnostic utility in identifying liver *** study was divided into 2 phases:marker selection by real-time quantitative PCR analysis of a small set of plasma samples,and marker validation with a large set of plasma samples from patients with chronic hepatitis B viral infections and healthy *** mouse model systems, D-galactosamine- and alcoholinduced liver injury,were also developed to evaluate whether differences in miRNA concentration were associated with various liver *** the miRNA candidates identified,miR-122 presented a disease severity-dependent change in plasma concentration in the patients and animal *** with an increase in aminotransferase activity in the blood,the change in miR-122 concentration appeared ***,this change was more specific for liver injury than for other organ damage and was more reliable, because the change was correlated with liver histologic ***:Our findings suggest that circulating miR-122 has potential as a novel,predictive,and reliable blood marker for viral-, alcohol-,and chemicalinduced liver injury.
背景与目的:靶向药物治疗(targeted drug therapy)逐渐成为是目前肿瘤治疗的重要手段。近年来的研究发现,磷脂酰丝氨酸(Phosphatidylserine,PS)是抗肿瘤治疗的重要靶点之-。膜联蛋白B1(annexin B1)是孙树汉教授克隆的一个新的膜联蛋白(A...
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背景与目的:靶向药物治疗(targeted drug therapy)逐渐成为是目前肿瘤治疗的重要手段。近年来的研究发现,磷脂酰丝氨酸(Phosphatidylserine,PS)是抗肿瘤治疗的重要靶点之-。膜联蛋白B1(annexin B1)是孙树汉教授克隆的一个新的膜联蛋白(Annexins)亚家族成员,对PS的结合具有很高的亲和力和特异性。蜂毒肽(melittin,MLT)是蜂毒的主要成分,具有很强的抗肿瘤作用。以肿瘤内皮细胞暴露的磷脂酰丝氨酸为"靶",以AnxB1为"导向分子",以蜂毒素为"抗肿瘤分子",构建基因工程靶向抗肿瘤药物mAnxB1-MLT。方法:利用PCR重叠区延伸法连接AnxB1-MLT,融合基因克隆至pGEX5T融合表达载体和pET-28a表达载体,转染大肠杆菌,分别就IPTG浓度、利福平、表达温度等条件进行优化。结果:融合蛋白AnxB1-MLT在pET-28a表达载体中无明显表达条带。37℃条件下,pGEX-5T表达质粒中高效表达,表达产物占菌体总蛋白的40%以上;IPTG、利福平对表达影响较小,但表达产物均为包涵体,不利于纯化。在22-25℃条件下诱导表达,虽然大肠杆菌生长较慢,但表达产物均为可溶性表达,且占菌体蛋白30%以上。利用GST亲和层析柱纯化,蛋白纯度大于90%。结论:成功构建了融合表达蛋白AnxB1-MLT的表达质粒,优化了诱导表达条件,获得了重组蛋白,为进一步通过动物模型研究其靶向抗肿瘤活性奠定了基础。
Background & Aims:The involvement of the hepatitis B virus X(HBx) protein in epigenetic modifications during hepatocarcinogenesis has been previously *** noncoding RNAs(IncRNAs),a kind
Background & Aims:The involvement of the hepatitis B virus X(HBx) protein in epigenetic modifications during hepatocarcinogenesis has been previously *** noncoding RNAs(IncRNAs),a kind
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