[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 k...
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[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 kb。测序结果与澳大利亚株猪囊尾蚴 Ag B序列有 99.8%的同源性 ,二者的氨基酸序列有 99.3%的同源性。 [结论 ]成功地克隆了猪囊尾蚴 Ag B基因 c DNA编码区。
The eukaryotic expression plasmid (pcDNA3 CEA)containing with the gene coding for the Carcinoembryonic antigen(CEA) had been gotten with RT PCR and gene recombination *** enzymolysis,ligation and other techniques,an e...
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The eukaryotic expression plasmid (pcDNA3 CEA)containing with the gene coding for the Carcinoembryonic antigen(CEA) had been gotten with RT PCR and gene recombination *** enzymolysis,ligation and other techniques,an eukaryotic coexpression plasmid(pcDNA3 CEA/HN)containing two expression unites of CEA and Newcastle disease virus HN gene that may have the function of immunoenhancement had been *** plasmid will lay a foundation for further researching CEA nucleiotide vaccine,adjuvant and their effect of special antitumor immune.
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