Recent increasing restrictions on animals have persuaded researchers to seek in vitro alternatives that could reduce the use of experimental animals and provide a unique methodology for the exploration of specific tox...
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Recent increasing restrictions on animals have persuaded researchers to seek in vitro alternatives that could reduce the use of experimental animals and provide a unique methodology for the exploration of specific toxicant mechanisms in *** various in vitro models have been reported,their application in toxicological studies has been limited due to various *** the present study,an improved three-dimensional rat testicular cell co-culture model was established by the addition of KSR to the culture medium instead of *** effects of KSR and FBS were assessed and followed by an assessment of the effects of BPA on somatic cell function and germ cell differentiation in vitro using this improved co-culture *** was found that the addition of KSR could enhance cell proliferation and germ cell meiosis,which confirmed the functional integrity of the co-culture ***,the effects of BPA on the in vitro model demonstrated that the model could successfully mimic in vivo testis physiology *** concluded that the modified model could be used to evaluate various chemicals on rat testis cells and investigate the specific mechanisms of toxicant activity in the testis in future studies.
The purpose of the present research is to further validate the application of the improved 3D rat testicular cell co-culture model to evaluate the effects of various reproductive toxic chemicals on the function of the...
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The purpose of the present research is to further validate the application of the improved 3D rat testicular cell co-culture model to evaluate the effects of various reproductive toxic chemicals on the function of the main somatic cells,as well as on spermatogonial cell differentiation and even spermatogenesis,and to investigate the specific toxicant mechanisms in testes with HZ 1006,a hydroxamate-based HD *** to the characteristics of HZ 1006,the appropriate exposure duration(8,16 and 24 days),dosage(0,3.125,6.25,12.5 and 25 μM) and toxic endpoints suitable for detection were selected in the *** results showed inhibition of cell proliferation,reduced testosterone level and decreased spermatogonial cell meiosis-specific gene expression,as well as decreased Ar protein level and Ar target gene PSA expression,accompanied by inhibition of Hdac6 expression after HZ 1006 exposure in the 3D rat testicular cell co-culture *** findings indicate that the improved 3D rat testicular cell co-culture model we have established has the potential to become a new testicular toxicity test system that can be used to test toxic characteristics and mechanisms of new compounds and has good application prospects,although it is necessary to do more research on the model.
Recent increasing restrictions on animals have persuaded researchers to seek in vitro alternatives that could reduce the use of experimental animals and provide unique methodologies for the exploration of specific tox...
详细信息
Recent increasing restrictions on animals have persuaded researchers to seek in vitro alternatives that could reduce the use of experimental animals and provide unique methodologies for the exploration of specific toxic mechanisms in follicles or *** various in vitro models have been reported,their application in toxicological studies has been limited for several *** the present study,an improved three-dimensional rat preantral follicle culture model was established by adding sodium alginate to culture *** effects of sodium alginate were assessed,followed by the assessment of the effects of BPA on in vitro follicle function and development with this improved culture *** addition of sodium alginate enhanced follicle development,oocyte maturation and hormone excretion,which confirmed the functional integrity of the culture ***,the effects of BPA on the in vitro model demonstrated that the model can successfully mimic in vivo follicle physiological *** concluded that the modified model can be used in the future for the evaluation of various chemicals on rat follicles and oocytes and investigation of the specific mechanisms of toxicant action in the female reproductive system,especially the ovaries.
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