Imbalance release of proangiogenic and antiangiogenic factor from placenta causes maternal endothelial dysfunction,the main manifestation of preeclampsia(PE).Recently,hydrogen sulfide(H2S)has been shown to have a pro-...
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Imbalance release of proangiogenic and antiangiogenic factor from placenta causes maternal endothelial dysfunction,the main manifestation of preeclampsia(PE).Recently,hydrogen sulfide(H2S)has been shown to have a pro-angiogenic effect and human placenta is able to produce *** therefore determined whether H2S is involved in the regulation of proangiogenic and antiangiogenic factors in placenta.H2S synthesis enzymes cystathionineβ-synthase(CBS)and cystathionineγ-lyase(CSE)expression was significantly downregulated in PE *** CSE and CBS positively correlated with the level of agiogenic factor vascular endothelial growth factor A(VEGFA)whereas inversely correlated to antiangiogenic factor soluble fms-like tyrosine kinase 1(s Flt-1)in placentas.H2S donor Na HS and precursor L-cysteine increased VEGFA whereas decreased s Flt-1 production in cultured placental explants as well as placental *** effects of H2S precursor L-cysteine could be reversed by si RNA of CBS and *** selective KATP inhibitor glybenclamide or specific PI3K,ERK and P38 inhibitors did not block the effects of H2S on VEGFA and s Flt-1.H2S affected the stability of VEGFA and s Flt-1 m RNA in placental *** of cells with Na HS or L-cysteine decreased the expression of mi R200c,mi R 20a and mi R20b which target VEGFA whereas increased the expression of mi R-133b which target s *** levels of mi R-200c,mi R 20a and mi R20b were upregulated whereasthe level of mi R-133b was downregulated in PE *** expression of mi R133b positively correlated to CBS and CSE level whereas mi R20a level inversely correlated to CBS and CSE expression in *** results suggest that H2S is an important factor that maintains the balance of proangiogenic factor VEGFA and antiangiogenic factor s Flt-1 in *** H2S generation in placenta contributes to the pathogenesis of PE.
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