检索结果-内蒙古大学图书馆

肿瘤防治研究 2020年第5期47卷 319-323页

作者：陈哲文石汉平缪明永余亚英张启瑜温州医科大学附属第二医院肝胆外科温州325000 首都医科大学附属北京世纪坛医院胃肠外科/临床营养科北京100038 第二军医大学基础医学部生物化学与分子生物学教研室上海200433 河南大学第一附属医院营养科开封475001 温州医科大学附属第一医院肝胆外科温州325000

目的探讨胰腺癌细胞在不同葡萄糖浓度下对甘露糖敏感度的变化与机制。方法 CCK-8细胞活性和增殖试剂盒检测在不同浓度葡萄糖与甘露糖作用下的胰腺癌细胞增殖情况,锥虫蓝染色测定细胞存活率,Western blot实验观察PMI酶表达的变化。生酮... 详细信息

目的探讨胰腺癌细胞在不同葡萄糖浓度下对甘露糖敏感度的变化与机制。方法 CCK-8细胞活性和增殖试剂盒检测在不同浓度葡萄糖与甘露糖作用下的胰腺癌细胞增殖情况,锥虫蓝染色测定细胞存活率,Western blot实验观察PMI酶表达的变化。生酮饮食联合甘露糖水溶液喂养荷瘤模型鼠验证实验结果。结果随着葡萄糖培养浓度降低,胰腺癌细胞增殖受到明显抑制,所需的甘露糖浓度也明显降低;高糖培养下甘露糖明显诱导PMI酶的表达,而在低糖下诱导效应减弱;生酮饮食具有降血糖、升血酮的作用,与甘露糖联合应用组的荷瘤鼠肿瘤抑制效果显著。结论低葡萄糖浓度增强甘露糖抑制肿瘤的作用,生酮饮食与甘露糖联合应用可提高抗肿瘤的疗效。

关键词：胰腺癌甘露糖葡萄糖肿瘤生酮饮食

来源：评论

学校读者我要写书评

暂无评论

The Histone Deacetylase4/Sp1/miR-200a Regulatory Network Contributes to Aberrant Histone Acetylation in Hepatocellular Carcinoma

The Histone Deacetylase4/Sp1/miR-200a Regulatory Network Con...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：袁继行杨富王芳孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

As an important epigenetic mechanism,histone acetylation modulates the transcription of many genes and plays important roles in hepatocellular carcinoma(HCC).Aberrations in histone acetylation have been observed in HCC,but the factors that contribute to the aberrations have not been fully ***(miRNAs),which are noncoding RNAs that regulate gene expression,are involved in important epigenetic *** this study,we determined that the miR-200a and the level ofhistone H3 acetylation at its promoter were reduced in human HCC tissues in comparison with adjacent noncancerous hepatic ***,our results suggested that the histone deacetylase 4(HDAC4)inhibited the expression of miR-200a and its promoter activity and reduced the histone H3 acetylation level at the mir-200a promoter through a Sp1-dependent ***,we observed that the miR-200a directly targeted the 3'untranslated region of the HDAC4 mRNA and repressed HDAC4's ***,the miR-200a ultimately induced its own transcription and increased the histone H3 acetylation level at its own *** targeting HDAC4,the miR-200a also induced the upregulation of total acetyl-histone H3 levels and increased the histone H3 acetylation level at the p21WAF/Cip1 ***,we determined that the miR-200a inhibited the proliferation and migration of HCC cells in vivo and in ***:Our findings suggest that the HDAC4/Sp1/miR-200a regulatory network induces the downregulation of the miR-200a and the upregulation of HDAC4 in *** a consequence,the downregulation of the miR-200a enhances the proliferation and migration of the HCC cells and induces aberrant histone acetylation in *** findings highlight a potential therapeutic approach in targeting the HDAC4/Sp1/miR-200a regulatory network for the treatment of HCC.

关键词： histone deacetylase 4 Sp1 miR-200a histone acetylation hepatocellular carcinoma

来源：评论

学校读者我要写书评

暂无评论

Epigenetic Activation of MiR-200Family Contributes to H19mediated Metastasis Suppression in Hepatocellular Carcinoma

Epigenetic Activation of MiR-200Family Contributes to H19med...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：张铃杨富孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

Recently,long non-coding RNA is thought to be contained in human genome as important regulatory transcripts in which we can identify far more previously *** serve as scaffolds associating with a protein and genome *** our recent study,we identified non-overlapping signatures of a small number of lncRNAs that are aberrantly expressed in human HBV-related HCC compared to paired pertitumoral tissues and among which lncRNA H19 was found to speculate a potential role in HCC *** from past studies has shown a strong association between H19 and carcinogenesis in several types of cancer including *** expression of H19 usually changes from monoallelic to biallelic in HCC through aberrant DNA methylation.

关键词： epigenetics histone acetylation hepatocellular carcinoma aggressiveness peritumoral tissues epithelial-to-mesenchymal transition

来源：评论

学校读者我要写书评

暂无评论

DNA Methylomes in CpG Island Methylator Phenotype(CIMP)Colon Cancer:new screening marks proposal

DNA Methylomes in CpG Island Methylator Phenotype(CIMP)Colon...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：颜宏利唐冠楠章意亮王玉招孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

DNA methylation is essential for normal development and is frequently altered in *** Island Methylator Phenotype(CIMP)is a subset of colorectal cancers was postulated to have a higher propensity for DNA ***,the validity of CIMP,its molecular basis,and its prognostic values are highly *** aims of this study were to(1)compare and characterize the unique features of DNA methylomes of CIMP with Non-CIMP colonrectal cancers;(2)propose a new panel of valuable markers for CIMP *** we utilized MBD-isolated Genome Sequencing(MiGS)to map the DNA methylomes of three sets of normal colon,non-CIMP colon cancers,and CIMP colon *** regions only robust hypermethylated in CIMP tumors were evaluated in 120 colorectal tumors by Bisulfite genomic sequencing or Methylation Specific PCR(MSP).We found 4,800 sites were hypermethylated in both non-CIMP and CIMP colon cancers when compared with normal *** those sites,318 sites were robust hypermethylated in CIMP tumors *** of these CpG islands regulates 122 key genes in tumorgenesis and progress.A panel of 5 new markers was proposed for CIMP screening in 120 consecutive colon samples,13.3%(16/120)were methylated in all 5 markers and 87.5%(14/16)were *** the markers described by Weisenberger et al.,22.5%(27/120)were methylated in all 5 markers and only 51.8%(14/27)were MSI *** are a subset of colorectal cancer has unique features in DNA *** genes only hypermethylated in CIMP tumors shed lights on exploration the mechanism of CIMP tumorgenesis and provide new targets for cancer *** new panel of markers facilitates CIMP screening in sporadic colorectal cancers.

关键词： colorectal cancer CpG island methylator phenotype MBD-isolated genome sequencing DNA methylome

来源：评论

学校读者我要写书评

暂无评论

LncRNA-LALR accelerates hepatocyte proliferation and cell cycle progression in liver regeneration by Wnt/β-Catenin signaling

LncRNA-LALR accelerates hepatocyte proliferation and cell cy...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：徐丹杨富毕海姗张铃周传传袁继行刘峰王芳孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

来源：评论

学校读者我要写书评

暂无评论

小鼠成胚胎成纤维细胞重编程为肝干细胞

小鼠成胚胎成纤维细胞重编程为肝干细胞

引用

中国细胞生物学学会2015年全国学术大会

作者：于兵何志颖攸璞韩庆旺陈费王敏君王欣胡以平第二军医大学基础医学部细胞生物学教研室上海200433 第二军医大学干细胞与医学研究中心上海200433 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室呼和浩特010070

谱系重编程是指将已经分化成熟的细胞转换为另一种成熟细胞或干/祖细胞,而不经过多能性干细胞阶段的重编程策略。本研究以谱系重编程的方法,用Hnf1β和Foxa3两个转录因子将小鼠胚胎成纤维细胞诱导转分化为诱导型肝干细胞(inducedhepatic...

谱系重编程是指将已经分化成熟的细胞转换为另一种成熟细胞或干/祖细胞,而不经过多能性干细胞阶段的重编程策略。本研究以谱系重编程的方法,用Hnf1β和Foxa3两个转录因子将小鼠胚胎成纤维细胞诱导转分化为诱导型肝干细胞(inducedhepatic stem cells,iHepSCs),iHepSCs表达肝干细胞的标志物(EpCAM,Lgr5,Sox9),但不表达成熟肝细胞的功能性标志物(G6pd,Tat,Aat)。

关键词：

来源：评论

学校读者我要写书评

暂无评论

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：黄金凤郭瀛军王坡赵晨曦唐冠楠王越刘音孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

Liver cancer is the fifth most common cancer in the world and the third most common cause of cancer-related ***,50% to 55% of cases of primary liver cancer are attributable to persistent hepatitis B virus(HBV)*** the pathogenesis of liver cancer is not very clear at *** the in-depth understanding of cancer,it is recognized that epigenetic modifications play crucial roles in the initiation and development of human *** is as much an epigenetic disease as it is a genetic disease,which together lead to the malignant transformation of cells caused by the activation of oncogene and the inactivation of tumor suppressor genes.

关键词： epigenetics hepatocellular carcinoma hepatitis B virus X protein long non-coding RNA vimentin

来源：评论

学校读者我要写书评

暂无评论

Hepatitis C-induced upregulation of miR-155promotes hepatocarcinogenesis by activating Wnt signaling

Hepatitis C-induced upregulation of miR-155promotes hepatoca...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：章意亮孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

Hepatitis C virus(HCV)infection induces chronic hepatic inflammation,which usually favors the initiation and progression of *** is also increasing clearly that microRNA-155 (miR-155)plays an important role in regulating both inflammation and ***,little is known about whether and how miR-155 provides the link between inflammation and *** we found that miR-155 levels were markedly increased after HCV infection and in hepatitis ***-155 expression was transcribed by NF-κB,and p300/CBP increased NF-κB-dependent miR-155 *** of miR-155 significantly inhibited hepatocytes apoptosis and promoted cells growth,whereas miR-155 inhibition induced G0/G1 *** miR-155 resulted in nuclear accumulation of β-catenin and a concomitant increase in cyclin D1,c-myc and ***-of-function and loss-of-function studies demonstrated that miR-155 promoted hepatocytes proliferation and tumorigenesis by increasing Wnt signaling in vitro and in vivo,and DKK1(Wnt pathway inhibitor)overexpression inhibited the biological role of miR-155 in ***,adenomatous polyposis coli(APC),which negatively regulates Wnt signaling,was identified as the direct and functional target of ***:Hepatitis C-induced upregulation of miR-155 promotes hepatocytes proliferation and tumorigenesis by activating Wnt *** present study provides a better understanding of the relationship between inflammation and tumor,and thus may be helpful in developing effective diagnosis and treatment strategies against HCV-HCC.

关键词： miR-155 wnt HCV HCC

来源：评论

学校读者我要写书评

暂无评论

Evi-1is a critical regulator of the HBx-induced aberrant transcription of long non-coding RNAs

Evi-1is a critical regulator of the HBx-induced aberrant tra...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：郭瀛军王越孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

The role of long non-coding RNAs(lncRNAs)in tumorigenesis is gradually being elucidated largely due to an increasing number of ***,the regulatory mechanism for lncRNA expression remains *** this study,we employed a systemic approach to analyze the key regulator of aberrantly expressed lncRNAs in HBV X protein(HBx)-transgenic mice,which is a well-proven mouse tumorigenesis model for hepatitis B virus-related hepatocellular *** a bioinformatics analysis of microarray data,ecotropic viral integration site 1(Evi-1)was identified as a key regulatory transcriptional factor contributing to the aberrant expression of lncRNAs in the livers of HBx-transgenic *** a consequence of the up-regulation of Evi-1,we also identified that an Evi-1-regulated lncRNA AK015487 contributed to hepatocyte proliferative activity and *** findings highlight a novel mechanism for hepatocellular carcinoma tumorigenesis through Evi-1 and its target *** addition,our study also provides a good model for investigation of the regulatory mechanism for lncRNA expression using a systemic approach.

关键词： hepatocarcinogenesis IncRNA Evi-1 HBx epigenetics

来源：评论

学校读者我要写书评

暂无评论

The"c-Myc/miR-15-16/FGF2"axis plays an important role in hypoxia-induced distant tumor metastasis

The"c-Myc/miR-15-16/FGF2"axis plays an important role in hyp...

引用

中华医学会2012年医学遗传学年会暨全国第十一次医学遗传学学术会议

作者：薛赓颜宏利张毅单石孙树汉第二军医大学基础医学部医学遗传学教研室上海200433

Previous studies have established the link between aberrant microRNA(miRNA)level and hypoxia in various ***,how these aberrant miRNAs induced by hypoxia modulate tumor progression is still *** this study,the miRNA patterns of colorectal carcinoma(CRC)cells in hypoxia were screened by a high-resolution miRNA microarray and the hypoxia-induced repression of miR-15-16 was *** repression was associated with higher tumor stage and poor prognosis in *** was further disclosed that the hypoxia-induced repression of miR-15-16 promoted angiogenesis and metastasis by the loss of post-transcriptional inhibition of fibroblast growth factor-2(FGF2),Moreover,the promoter of the host gene (deleted in lymphocytic leukemia 2,DLEU2)of the miR-15-16 cluster was mapped and three Myc/Max binding sites responding in hypoxia were ***,the transcriptional repression of c-Myc to miR-15-16 via these Myc/Max binding sites in hypoxia was ***,we verified that the functional "c-Myc/miR-15-16/FGF2"pathway would play an important role in hypoxia-induced distant tumor *** a word,c-Myc-mediated repression of the miR-15-16 cluster in hypoxia promotes tumor angiogenesis and distant metastasis by upregulating *** study provides a better understanding of the coping mechanisms of microRNAs in response to hypoxia and may be helpful in developing more effective prognostic markers or treatment targets for solid tumors.

关键词： c-Myc fibroblast growth factor-2 hypoxia metastasis miR-15-1

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：