目的探讨高压氧暴露后小鼠低氧耐受状态的变化。方法昆明种小白鼠123只随机均分为高压氧暴露(HBO)组、高压空气对照(HBA)组、空白对照(NBA)组,经过5 d处理后每组取25只检测动物在低氧条件下的存活时间,另外每组16只进行力竭游泳耐受时间检测。结果HBO组中位生存时间(108 m in)较NBA组(57 m in)明显延长,HBO与NBA两条生存曲线的差异有统计学意义(Log-rank检验,P<0.01)。HBO组低氧力竭游泳耐受时间[(45.19±17.27)m in]较NBA组[(20.96±11.30)m in]明显延长(P<0.01)。结论高压氧暴露可改善小鼠低氧耐受状态。
Objective To examine the effect of neuropeptide Y （NPY） on TGF-β1 production in RAW264.7 macrophages. Methods Enzyme linked immunosorbent assay （ELISA） was used to detect TGF-β1 production. Cell counting kit 8 ...
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Objective To examine the effect of neuropeptide Y （NPY） on TGF-β1 production in RAW264.7 macrophages. Methods Enzyme linked immunosorbent assay （ELISA） was used to detect TGF-β1 production. Cell counting kit 8 （CCK-8） was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85. Results NPY treatment could promote TGF-β1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-β 1 production induced by NPY could be abolished by wortrnannin pretreatment. Conclusion NPY may elicit TGF-β production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.
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