Objective:.we have investigated the cellular and humoral immune responses in ex vivo and immune protection in vivo of immunized mice by DNA vaccine encoding TXR1. Methods: The c DNA encoded TXR1 was acquired by PCR fr...
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Objective:.we have investigated the cellular and humoral immune responses in ex vivo and immune protection in vivo of immunized mice by DNA vaccine encoding TXR1. Methods: The c DNA encoded TXR1 was acquired by PCR from a full-length *** inserted into the sequence between the luminal and transmem-brane domain of human lysosome-associated membrane protein-1(LAMP-1) c DNA for the construction of a novel DNA vaccine, named p VAX-L/TXR1. Other two plasmids were constructed respectively,i.e. p VAX-TXR1 and p ***,We tested specific antibody production in serum two weeks after each immunization by ELISA and neutralizing activity of antibody against TXR1 by the cell microculture neutralization assay for humoral response evaluation. At the same time, we measured the peptide-specific IFN-γ secretion by ELISpot and specific CTL cytotoxicity against P815 cells which were loaded with NP overlaping peptide-pool by LDH assay to evaluate cellular immune response. Results: The mice were immunized subcutaneously three times at the base of tail with four plasmids in each group respectively. Immune responses were analyzed by ELISPOT, Cytotoxicity and ELISA. ELISpot data showed that single peptide 6,9,11 could stimulate T cells secreting IFN-γ in p VAX1-LAMP/TXR1 group. Moreover, the number of specific T cells and immune response effects significantly increased comparing to the p VAX1-LAMP control group(P <0.05).In Cytotoxicity assay, when the effect to target ratio was 40: l,the killing effect of group p VAX1-LAMP/TXR1 was1.4 times as the group p VAX1-TXR1. Indirect ELISA results showed that group p VAX1-LAMP/TXR1 could produce specific antibodies against *** the increasement in the frequency of immunization, the antibody concentration gradually increases. Our experiments clarified that CD4+ T cells were effectively activated by the LAMP targeting and the humoral and cellular immune responses were enhanced. Conclusions: In summary, TXR1 showed a strong immunogenicity t
Objective: we have investigated the cellular and humoral immune responses in ex vivo and immune protection in vivo of immunized mice by DNA vaccine encoding TXR1.
Objective: we have investigated the cellular and humoral immune responses in ex vivo and immune protection in vivo of immunized mice by DNA vaccine encoding TXR1.
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