为了探讨土壤线虫在人工林生态系统中的功能及其对环境变化的响应。该研究利用18S r DNA的宏基因组比较了湖南省攸县不同发育阶段(3年、13年、26年)以及不同栽培代数(1代、2代、3代)杉木人工林的土壤线虫的群落结构及多样性。结果表明:...
详细信息
为了探讨土壤线虫在人工林生态系统中的功能及其对环境变化的响应。该研究利用18S r DNA的宏基因组比较了湖南省攸县不同发育阶段(3年、13年、26年)以及不同栽培代数(1代、2代、3代)杉木人工林的土壤线虫的群落结构及多样性。结果表明:(1)测序共获得了2 111条隶属于线虫动物门的序列。经聚类后产生了27个OTUs。主要类群有Prismatolaimidae、Monhysteridae、Alaimidae、Qudsianematidae、Criconematidae、Plectidae它们分别占总序列数的31%、15%、11%、8%、6%、5%,其中Prismatolaimidae在所有样地中均占绝对优势,Aphelenchidae、Cephalobidae、Criconematidae、Rhabdolaimidae、Tylenchidae这5个类群的相对丰度在各样地间具有显著性差异(p<0.05);(2)冗余分析(RDA)发现所选的5块样地的线虫的群落结构有着明显的差异,FRC,SYC,TYC三块样地的群落结构较为相似。FYC与FRC之间差异性最大;(3)6种不同的多样性指数的单因素方差分析发现,各样地的多样性指数之间没有达到显著性差异。说明长期种植单一杉木人工林能够改变土壤线虫的群落组成,但并没有改变种群的多样性。土壤线虫中存在种的冗余现象。
Tar spot of Dalbergia odorifera, caused by Phyllachora dalbergiicola, is a common disease, and se-riously affected the rate of photosynthesis. Here we developed a species-specific Nested-PCR approach for rapidand accu...
详细信息
Tar spot of Dalbergia odorifera, caused by Phyllachora dalbergiicola, is a common disease, and se-riously affected the rate of photosynthesis. Here we developed a species-specific Nested-PCR approach for rapidand accurate detection of P. dalbergiicola, based on the differences in internal transcribed spacer （ITS） se-quences of P. dalbergiicola and another P. spp., an endophytic fungus, from which a pair of species-specificprimers P1/P2 （P1 ： 5＂-CGAGGTCAGAATCAAACG-3＂, P2： 5＂-TGAAGAACGCAGCGAAAT-3＂）, was designed.P1/P2 amplified only a unique 273 bp band from the genomic DNA of P. dalbergiicola. A Nested-PCR proce-dure using ITS4/ITS5 as the first-round primers, followed by P1/P2 primers, increased detection sensitivity10 000 fold to 100 ag. Using the Fast DNA-kit to extract DNA from the diseased plant tissues, the detection ofthe pathogen by Nested-PCR assay could be completed within I day. The results suggested that the PCR-basedmethods here could simplify both plant disease diagnosis and pathogen detection.
暂无评论