In this study, with Jiuzihe poria as the main raw material, the effect of alkali concentration, extraction time and solid-liquid ratio on the extraction rate of alkali-soluble polysaccharide was investigated, and the ...
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In this study, with Jiuzihe poria as the main raw material, the effect of alkali concentration, extraction time and solid-liquid ratio on the extraction rate of alkali-soluble polysaccharide was investigated, and the processing technology was optimized with orthogonal experiment. The results showed that the optimum extraction process for alkali-soluble polysacchadde from poria was as follows： solid-liquid ratio of 1：50, alkali concentration of 0.5 mol/L, and extraction time of 8 h.
From the NCBI database, 81,518 Lactuca sativa EST sequences were downloaded, from which 61,757 non-redundant sequences were obtained. In total, 2040 SSR loci were identified, with the frequency of 3.3%. Trinucleotide ...
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From the NCBI database, 81,518 Lactuca sativa EST sequences were downloaded, from which 61,757 non-redundant sequences were obtained. In total, 2040 SSR loci were identified, with the frequency of 3.3%. Trinucleotide repeat was dominant, followed by dinucieotide and hexanucleotide repeats. Among 181 types of repeat motifs, （AGA）n and （ATG）n were the dominant repeat motif types, taking up 12.01% and 3.53%, respectively. Totally, four material-special EST-SSR markers were characterized. This study enriches molecular markers of L. sativa, and also lays a theoretical foundation for following germplasm identification, molecular marker assisted breeding and genetic map construction.
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