目的:探讨DC的抗肿瘤免疫功能是否与其基因位点有关。方法:选择19株基因型明确的BXD纯系小鼠的树突状细胞(DC),MTT法和ELISA分析对DC吞噬肿瘤细胞的作用;肿瘤细胞诱导:DC产生IL-12的能力;经肿瘤细胞刺激后的DC对T细胞增殖和:IFN-γ分泌的影响进行测定。用流式细胞仪检测:DC表面抗原标志CD80和CD54。有关数据用MapManagerQTX and WebQTL软件对DC抗肿瘤免疫的基因位点进行分析。结果:DC刺激T细胞增殖的作用很大程度取决于BXD各系的遗传差异。DC吞噬肿瘤细胞及诱导IL-12产生的能力都与DC刺激T细胞的增殖相一致(P<0.01)。基因位点定量分析(QTL)分析在第6和第13号染色体上有2个位点可能与DC的肿瘤抗原提呈加工功能和T细胞毒作用有关。结论:不同系的BXD小鼠,其DC的抗原提呈、加工能力不同。DC对肿瘤细胞生长的抑制与IL-12的诱导产生和DC表面共刺激分子的表达呈平行。其有关基因位点分别于第6和第13号染色体上。
Objective To search novel genes or pathways involved in the recovery process after restraint stress in rats. Methods We compared the hypothalamus transcriptional profiles of two different recovery patterns （fast reco...
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Objective To search novel genes or pathways involved in the recovery process after restraint stress in rats. Methods We compared the hypothalamus transcriptional profiles of two different recovery patterns （fast recovery vs slow recovery） from restraint stress in rats using oligonucleotide microarray, the recovery pattern was determined by the decrement of plasma adrenocorticotropic-hormone （ACTH） and corticosterone levels during one hour recovery period after stress. A real-time quantitative RT-PCR was applied to validate the differential expressed genes. Results Analysis of the microarray data showed that most of genes were not differentially expressed between fast recovery group and slow recovery group. Among the differentially expressed genes we found that talin, together with serine/threonine protein phosphatase PPl-beta catalytic subunit （PP-1B） and integrin α-6 precursor （VLA-6） genes, were at least 1.5 fold upregulated in the fast recovery group, while junctional adhesion molecule 1 （F11r） was 1.5 fold down-regulated in the fast recovery group. Conclusion The results implied that integrin signaling pathway may be involved in the recovery from restraint stress in rats. The present study provided a global overview of hypothalamus transcriptional profiles during the process of recovery from the restraint stress in rats. The integrin signaling pathway seems to be involved in the recovery process, which deserves further study to clarify the integrin-mediated recovery mechanism after restraint stress.
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